@article{f8357c1c71f0444f95639c1381d90321,
title = "Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants",
abstract = "Caenorhabditis elegans homologues of the retinoblastoma (Rb) tumour suppressor complex specify cell lineage during development. Here we show that mutations in Rb pathway components enhance RNA interference (RNAi) and cause somatic cells to express genes and elaborate perinuclear structures normally limited to germline-specific P granules. Furthermore, particular gene inactivations that disrupt RNAi reverse the cell lineage transformations of Rb pathway mutants. These findings suggest that mutations in Rb pathway components cause cells to revert to patterns of gene expression normally restricted to germ cells. Rb may act by a similar mechanism to transform mammalian cells.",
author = "Duo Wang and Scott Kennedy and Darryl Conte and Kim, {John K.} and Gabel, {Harrison W.} and Kamath, {Ravi S.} and Mello, {Craig C.} and Gary Ruvkun",
note = "Funding Information: Acknowledgements We thank E. C. Andersen, W. G. Kelly, N. Dyson and V. Reineke for discussions; T. Sijen for reagents; H. Y. Mak for the unpublished tub-1::gfp transgene; J. Ahringer and M. Vidal for the feeding RNAi bacteria clones; S. Fischer for constructing the hand-picked RNAi library; S. Strome for the anti-PGL-1 antibodies; and E. C. Andersen, H. R. Horvitz, C. P. Hunter, F. Palladino, the Caenorhabditis Genetics Center and C. elegans Gene Knockout Consortium for some of the strains.",
year = "2005",
month = jul,
day = "28",
doi = "10.1038/nature04010",
language = "English",
volume = "436",
pages = "593--597",
journal = "Nature",
issn = "0028-0836",
number = "7050",
}