@article{6d616d2fafc4416f80a885eb54573867,
title = "Small nucleolar RNAs U32a, U33, and U35a are critical mediators of metabolic stress",
abstract = "Lipotoxicity is a metabolic stress response implicated in the pathogenesis of diabetes complications and has been shown to involve lipid-induced oxidative stress. To elucidate the molecular mechanisms of lipotoxicity, we used retroviral promoter trap mutagenesis to isolate a cell line that is resistant to lipotoxic and oxidative stress. We show that loss of three box C/D small nucleolar RNAs (snoRNAs) encoded in the ribosomal protein L13a (rpL13a) locus is sufficient to confer resistance to lipotoxic and oxidative stress in vitro and prevents the propagation of oxidative stress in vivo. Our results provide evidence for a previously unappreciated, non-canonical role for box C/D snoRNAs as regulators of metabolic stress response pathways in mammalian cells.",
author = "Michel, {Carlos I.} and Holley, {Christopher L.} and Scruggs, {Benjamin S.} and Rohini Sidhu and Brookheart, {Rita T.} and Listenberger, {Laura L.} and Behlke, {Mark A.} and Ory, {Daniel S.} and Schaffer, {Jean E.}",
note = "Funding Information: This work was supported by grants to J.E.S. from the National Institutes of Health (NIH; DK064989) and the Burroughs Wellcome Foundation (1005935), to C.I.M. from the NIH (DK077577), and to R.T.B. from the NIH (DK077583) and to D.S.O. from NIH (HL103001). Support from the Washington University Diabetes Research Training Center (DK020579), the Washington University Diabetic Cardiovascular Disease Metabolomics Facility, NIH T32 HL07081, and NIH T32 HL07275 is also acknowledged. M.A.B. is employed by Integrated DNA Technologies (IDT), which offers oligonucleotides for sale similar to some of the compounds described in the manuscript. IDT is, however, not a publicly traded company, and M.A.B. does not own any shares/equity in IDT. ",
year = "2011",
month = jul,
day = "6",
doi = "10.1016/j.cmet.2011.04.009",
language = "English",
volume = "14",
pages = "33--44",
journal = "Cell metabolism",
issn = "1550-4131",
number = "1",
}