Slow conformational dynamics in the cystoviral RNA-directed RNA polymerase P2: Influence of Substrate nucleotides and template RNA

Zhen Ren, Ranajeet Ghose

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The RNA-directed RNA polymerase P2 from cystovirus φ6 catalyzes the de novo synthesis of positive and negative strands of the viral double-stranded RNA genome. P2 is mobile on the slow, microsecond to millisecond time scale with various motional modes, putatively assisting in RNA translocation and catalysis. Here we investigate the influence of the extreme 3′-end sequence of the single-stranded RNA templates and the nature of the substrate nucleotide triphosphates on these motional modes using multiple-quantum NMR spectroscopy. We find that P2, in the presence of templates bearing the proper genomic 3′-ends or the preferred initiation nucleotide, displays unique dynamic signatures that are different from those in the presence of nonphysiological templates or substrates. This suggests that dynamics may play a role in the fidelity of recognition of the correct substrates and template sequences to initiate RNA polymerization.

Original languageEnglish
Pages (from-to)1875-1884
Number of pages10
JournalBiochemistry
Volume50
Issue number11
DOIs
StatePublished - Mar 22 2011

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