TY - JOUR
T1 - Size fractionation of anionic oligosaccharides and glycopeptides by high-performance liquid chromatography
AU - Mellis, Scott J.
AU - Baenziger, Jacques U.
N1 - Funding Information:
’ This investigation was supportedb y Grant RO 1 CA 2 1923 awarded by the National Cancer Institute, DHEW. J.U.B. is a recipient of a Research Career Development Award, Grant 5 K04 CA0867 1, from the National Cancer Institute, DHEW. S.J.M. is a Medical Scientist Trainee supported by Grant GM07200 from the National Institute of General Medical Sciences. This study was also supported by the following companies: Brown & Williamson Tobacco Corporation, Philip Morris Incorporated, R. J. Reynolds Tobacco Company, and United States Tobacco Company.
PY - 1983/10/15
Y1 - 1983/10/15
N2 - A rapid method for the fractionation of anionic oligosaccharide and glycopeptide species on the basis of net carbohydrate content utilizing high-performance liquid chromatography has been developed. Amine-bearing bonded-phase columns are eluted with a mobile phase consisting of a water:acetonitrile gradient containing 3% acetic acid titrated to pH 5.5 with triethylamine. Phosphorylated and sialylated oligosaccharides within various charge classes differing in their hexose or hexosamine contents but bearing the same number of anionic species can be resolved without prior removal of the anionic moieties. Glycopeptides containing at least as many as six amino acids are also well fractionated on the basis of carbohydrate content. A variety of detection methods may be used and sensitivity in the subnanomole range is possible with fluorescent or radiolabeling techniques. This method offers a significant improvement in the rapidity and resolution attainable for the size fractionation of anionic complex carbohydrates.
AB - A rapid method for the fractionation of anionic oligosaccharide and glycopeptide species on the basis of net carbohydrate content utilizing high-performance liquid chromatography has been developed. Amine-bearing bonded-phase columns are eluted with a mobile phase consisting of a water:acetonitrile gradient containing 3% acetic acid titrated to pH 5.5 with triethylamine. Phosphorylated and sialylated oligosaccharides within various charge classes differing in their hexose or hexosamine contents but bearing the same number of anionic species can be resolved without prior removal of the anionic moieties. Glycopeptides containing at least as many as six amino acids are also well fractionated on the basis of carbohydrate content. A variety of detection methods may be used and sensitivity in the subnanomole range is possible with fluorescent or radiolabeling techniques. This method offers a significant improvement in the rapidity and resolution attainable for the size fractionation of anionic complex carbohydrates.
UR - http://www.scopus.com/inward/record.url?scp=0021061759&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(83)90320-2
DO - 10.1016/0003-2697(83)90320-2
M3 - Article
C2 - 6650829
AN - SCOPUS:0021061759
VL - 134
SP - 442
EP - 449
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -