Site-specific molecular diffusion in articular cartilage measured using fluorescence recovery after photobleaching

Holly A. Leddy, Farshid Guilak

Research output: Contribution to journalArticle

130 Scopus citations

Abstract

A study is presented on site-specific molecular diffusion in articular cartilage measured using fluorescence recovery after photobleaching (FRAP). The diffusion coefficients measured using FRAP exhibited an inverse size dependence and were in general agreement with those measured using other techniques. It was observed that diffusion coefficients for all molecules varied significantly with depth in a manner that depended upon the size of the diffusing molecule.

Original languageEnglish
Pages (from-to)753-760
Number of pages8
JournalAnnals of biomedical engineering
Volume31
Issue number7
DOIs
StatePublished - Aug 11 2003
Externally publishedYes

Keywords

  • Collagen
  • Confocal microscopy
  • Dextran
  • FRAP
  • Osteoarthritis
  • Proteoglycan

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