Single tether formation from unstimulated microvascular endothelial cells

G. Girdhar; S. J. Oswald; J. Xu; J. Y. Shao

Single tether formation from unstimulated microvascular endothelial cells

G. Girdhar, S. J. Oswald, J. Xu, J. Y. Shao

Research output: Contribution to journal › Conference article › peer-review

Abstract

Endothelial cells are actively involved in recruitment of leukocytes through receptor mediated cell adhesion. Previous studies indicate that tether formation is vital to a sustained receptor-ligand interaction subject to shear stresses encountered under physiological conditions [8]. However, limited information exists on the contribution of membrane properties of these cells towards a stable rolling interaction with leukocytes. We therefore propose to estimate the Intrinsic Tether Force (F₀) and Membrane Viscosity (η_eff) for single tether formation from attached and suspended unstimulated microvascular endothelial cells (MECs) using anti-CD29 and anti-CD31 antibody-coated beads. The analysis predicts a linear relationship between the tether force and velocity as has been shown previously [1]. Further, comparable estimates of the intrinsic parameters (F₀ and η_eff) using anti-CD31 and anti-CD29 coated beads also support the hypothesis that tether formation from MECs is independent of the surface receptor type.

Original language	English
Pages (from-to)	895-896
Number of pages	2
Journal	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume	2
State	Published - 2002
Event	Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States Duration: Oct 23 2002 → Oct 26 2002

Keywords

Intrinsic
Leukocytes
Rolling
Viscosity

Cite this

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title = "Single tether formation from unstimulated microvascular endothelial cells",

abstract = "Endothelial cells are actively involved in recruitment of leukocytes through receptor mediated cell adhesion. Previous studies indicate that tether formation is vital to a sustained receptor-ligand interaction subject to shear stresses encountered under physiological conditions [8]. However, limited information exists on the contribution of membrane properties of these cells towards a stable rolling interaction with leukocytes. We therefore propose to estimate the Intrinsic Tether Force (F0) and Membrane Viscosity (ηeff) for single tether formation from attached and suspended unstimulated microvascular endothelial cells (MECs) using anti-CD29 and anti-CD31 antibody-coated beads. The analysis predicts a linear relationship between the tether force and velocity as has been shown previously [1]. Further, comparable estimates of the intrinsic parameters (F0 and ηeff) using anti-CD31 and anti-CD29 coated beads also support the hypothesis that tether formation from MECs is independent of the surface receptor type.",

keywords = "Intrinsic, Leukocytes, Rolling, Viscosity",

author = "G. Girdhar and Oswald, {S. J.} and J. Xu and Shao, {J. Y.}",

year = "2002",

language = "English",

volume = "2",

pages = "895--896",

journal = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

issn = "0589-1019",

note = "Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) ; Conference date: 23-10-2002 Through 26-10-2002",

}

TY - JOUR

T1 - Single tether formation from unstimulated microvascular endothelial cells

AU - Girdhar, G.

AU - Oswald, S. J.

AU - Xu, J.

AU - Shao, J. Y.

PY - 2002

Y1 - 2002

N2 - Endothelial cells are actively involved in recruitment of leukocytes through receptor mediated cell adhesion. Previous studies indicate that tether formation is vital to a sustained receptor-ligand interaction subject to shear stresses encountered under physiological conditions [8]. However, limited information exists on the contribution of membrane properties of these cells towards a stable rolling interaction with leukocytes. We therefore propose to estimate the Intrinsic Tether Force (F0) and Membrane Viscosity (ηeff) for single tether formation from attached and suspended unstimulated microvascular endothelial cells (MECs) using anti-CD29 and anti-CD31 antibody-coated beads. The analysis predicts a linear relationship between the tether force and velocity as has been shown previously [1]. Further, comparable estimates of the intrinsic parameters (F0 and ηeff) using anti-CD31 and anti-CD29 coated beads also support the hypothesis that tether formation from MECs is independent of the surface receptor type.

AB - Endothelial cells are actively involved in recruitment of leukocytes through receptor mediated cell adhesion. Previous studies indicate that tether formation is vital to a sustained receptor-ligand interaction subject to shear stresses encountered under physiological conditions [8]. However, limited information exists on the contribution of membrane properties of these cells towards a stable rolling interaction with leukocytes. We therefore propose to estimate the Intrinsic Tether Force (F0) and Membrane Viscosity (ηeff) for single tether formation from attached and suspended unstimulated microvascular endothelial cells (MECs) using anti-CD29 and anti-CD31 antibody-coated beads. The analysis predicts a linear relationship between the tether force and velocity as has been shown previously [1]. Further, comparable estimates of the intrinsic parameters (F0 and ηeff) using anti-CD31 and anti-CD29 coated beads also support the hypothesis that tether formation from MECs is independent of the surface receptor type.

KW - Intrinsic

KW - Leukocytes

KW - Rolling

KW - Viscosity

UR - http://www.scopus.com/inward/record.url?scp=0036911166&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0036911166

SN - 0589-1019

VL - 2

SP - 895

EP - 896

JO - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

JF - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

T2 - Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)

Y2 - 23 October 2002 through 26 October 2002

ER -

Single tether formation from unstimulated microvascular endothelial cells

Abstract

Keywords

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