Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy

Richard K.P. Benninger; William J. Ashby; Elisabeth A. Ring; David W. Piston

doi:10.1364/OL.33.002895

Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy

Richard K.P. Benninger, William J. Ashby, Elisabeth A. Ring, David W. Piston

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

We present the use and characterization of a photon-counting detector for increased sensitivity at low signal levels in fluorescence laser scanning microscopy (LSM). Conventional LSM photomultiplier tube detectors utilize analog current integration and thus suffer from excessive noise at low signal levels, introduced during current measurement. In this Letter we describe the implementation of a fast single-photon-counting (SPC) detector on a conventional two-photon laser scanning microscope and detail its use in imaging low fluorescence intensities. We show that for a low photon flux, the SPC detector is shot-noise limited and thus provides increased detection sensitivity compared with analog current integration.

Original language	English
Pages (from-to)	2895-2897
Number of pages	3
Journal	Optics Letters
Volume	33
Issue number	24
DOIs	https://doi.org/10.1364/OL.33.002895
State	Published - Dec 15 2008

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abstract = "We present the use and characterization of a photon-counting detector for increased sensitivity at low signal levels in fluorescence laser scanning microscopy (LSM). Conventional LSM photomultiplier tube detectors utilize analog current integration and thus suffer from excessive noise at low signal levels, introduced during current measurement. In this Letter we describe the implementation of a fast single-photon-counting (SPC) detector on a conventional two-photon laser scanning microscope and detail its use in imaging low fluorescence intensities. We show that for a low photon flux, the SPC detector is shot-noise limited and thus provides increased detection sensitivity compared with analog current integration.",

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AU - Ashby, William J.

AU - Ring, Elisabeth A.

AU - Piston, David W.

PY - 2008/12/15

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AB - We present the use and characterization of a photon-counting detector for increased sensitivity at low signal levels in fluorescence laser scanning microscopy (LSM). Conventional LSM photomultiplier tube detectors utilize analog current integration and thus suffer from excessive noise at low signal levels, introduced during current measurement. In this Letter we describe the implementation of a fast single-photon-counting (SPC) detector on a conventional two-photon laser scanning microscope and detail its use in imaging low fluorescence intensities. We show that for a low photon flux, the SPC detector is shot-noise limited and thus provides increased detection sensitivity compared with analog current integration.

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ER -

Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy

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