Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy

Richard K.P. Benninger, William J. Ashby, Elisabeth A. Ring, David W. Piston

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We present the use and characterization of a photon-counting detector for increased sensitivity at low signal levels in fluorescence laser scanning microscopy (LSM). Conventional LSM photomultiplier tube detectors utilize analog current integration and thus suffer from excessive noise at low signal levels, introduced during current measurement. In this Letter we describe the implementation of a fast single-photon-counting (SPC) detector on a conventional two-photon laser scanning microscope and detail its use in imaging low fluorescence intensities. We show that for a low photon flux, the SPC detector is shot-noise limited and thus provides increased detection sensitivity compared with analog current integration.

Original languageEnglish
Pages (from-to)2895-2897
Number of pages3
JournalOptics Letters
Volume33
Issue number24
DOIs
StatePublished - Dec 15 2008
Externally publishedYes

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