Single molecule transcription elongation

Eric A. Galburt; Stephan W. Grill; Carlos Bustamante

doi:10.1016/j.ymeth.2009.04.021

Single molecule transcription elongation

Eric A. Galburt, Stephan W. Grill, Carlos Bustamante

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Single molecule optical trapping assays have now been applied to a great number of macromolecular systems including DNA, RNA, cargo motors, restriction enzymes, DNA helicases, chromosome remodelers, DNA polymerases and both viral and bacterial RNA polymerases. The advantages of the technique are the ability to observe dynamic, unsynchronized molecular processes, to determine the distributions of experimental quantities and to apply force to the system while monitoring the response over time. Here, we describe the application of these powerful techniques to study the dynamics of transcription elongation by RNA polymerase II from Saccharomyces cerevisiae.

Original language	English
Pages (from-to)	323-332
Number of pages	10
Journal	Methods
Volume	48
Issue number	4
DOIs	https://doi.org/10.1016/j.ymeth.2009.04.021
State	Published - Aug 2009

Keywords

Backtracking
Diffusion
Molecular motor
Optical trapping
RNA polymerase II
Single molecule
TFIIS
Transcriptional pausing

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10.1016/j.ymeth.2009.04.021

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title = "Single molecule transcription elongation",

abstract = "Single molecule optical trapping assays have now been applied to a great number of macromolecular systems including DNA, RNA, cargo motors, restriction enzymes, DNA helicases, chromosome remodelers, DNA polymerases and both viral and bacterial RNA polymerases. The advantages of the technique are the ability to observe dynamic, unsynchronized molecular processes, to determine the distributions of experimental quantities and to apply force to the system while monitoring the response over time. Here, we describe the application of these powerful techniques to study the dynamics of transcription elongation by RNA polymerase II from Saccharomyces cerevisiae.",

keywords = "Backtracking, Diffusion, Molecular motor, Optical trapping, RNA polymerase II, Single molecule, TFIIS, Transcriptional pausing",

author = "Galburt, {Eric A.} and Grill, {Stephan W.} and Carlos Bustamante",

note = "Funding Information: We thank Mikhail Kashlev and Maria Kireeva for making the described work possible. We also thank Anna Wiedmann, Juan Parrondo and Steve Smith for invaluable contributions along the way. The work described was funded by an NIH Grant GM-32543 (C.J.B.), a Jane Coffin Childs Postdoctoral Fellowship (E.A.G.) and by a Helen Hay Whitney Postdoctoral Fellowship (S.W.G.). ",

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doi = "10.1016/j.ymeth.2009.04.021",

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AU - Galburt, Eric A.

AU - Grill, Stephan W.

AU - Bustamante, Carlos

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N2 - Single molecule optical trapping assays have now been applied to a great number of macromolecular systems including DNA, RNA, cargo motors, restriction enzymes, DNA helicases, chromosome remodelers, DNA polymerases and both viral and bacterial RNA polymerases. The advantages of the technique are the ability to observe dynamic, unsynchronized molecular processes, to determine the distributions of experimental quantities and to apply force to the system while monitoring the response over time. Here, we describe the application of these powerful techniques to study the dynamics of transcription elongation by RNA polymerase II from Saccharomyces cerevisiae.

AB - Single molecule optical trapping assays have now been applied to a great number of macromolecular systems including DNA, RNA, cargo motors, restriction enzymes, DNA helicases, chromosome remodelers, DNA polymerases and both viral and bacterial RNA polymerases. The advantages of the technique are the ability to observe dynamic, unsynchronized molecular processes, to determine the distributions of experimental quantities and to apply force to the system while monitoring the response over time. Here, we describe the application of these powerful techniques to study the dynamics of transcription elongation by RNA polymerase II from Saccharomyces cerevisiae.

KW - Backtracking

KW - Diffusion

KW - Molecular motor

KW - Optical trapping

KW - RNA polymerase II

KW - Single molecule

KW - TFIIS

KW - Transcriptional pausing

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SN - 1046-2023

VL - 48

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JO - Methods

JF - Methods

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ER -

Single molecule transcription elongation

Abstract

Keywords

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