Single molecule transcription elongation

Eric A. Galburt, Stephan W. Grill, Carlos Bustamante

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Single molecule optical trapping assays have now been applied to a great number of macromolecular systems including DNA, RNA, cargo motors, restriction enzymes, DNA helicases, chromosome remodelers, DNA polymerases and both viral and bacterial RNA polymerases. The advantages of the technique are the ability to observe dynamic, unsynchronized molecular processes, to determine the distributions of experimental quantities and to apply force to the system while monitoring the response over time. Here, we describe the application of these powerful techniques to study the dynamics of transcription elongation by RNA polymerase II from Saccharomyces cerevisiae.

Original languageEnglish
Pages (from-to)323-332
Number of pages10
JournalMethods
Volume48
Issue number4
DOIs
StatePublished - Aug 2009

Keywords

  • Backtracking
  • Diffusion
  • Molecular motor
  • Optical trapping
  • RNA polymerase II
  • Single molecule
  • TFIIS
  • Transcriptional pausing

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