Single molecule profiling of alternative pre-mRNA splicing

Jun Zhu, Jay Shendure, Robi D. Mitra, George M. Church

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

Alternative pre-messenger RNA splicing is an important mechanism for generating protein diversity and may explain in part how mammalian complexity arises from a surprisingly small complement of genes. Here, we describe "digital polony exon profiling," a single molecule-based technology for studying complex alternative pre-messenger RNA splicing. This technology allows researchers to monitor the combinatorial diversity of exon inclusion in individual transcripts. A minisequencing strategy provides single nucleotide resolution, and the digital nature of the technology allows quantitation of individual splicing variants. Digital polony exon profiling can be used to investigate the physiological and pathological roles of alternately spliced messenger RNAs, as well as the mechanisms by which these messenger RNAs are produced.

Original languageEnglish
Pages (from-to)836-838
Number of pages3
JournalScience
Volume301
Issue number5634
DOIs
StatePublished - Aug 8 2003

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