Single molecule fluorescence study of DNA helicase activity

T. Ha; H. P. Babcock; W. Cheng; T. M. Lohman; S. Chu

doi:10.1109/cleo.2000.907430

Single molecule fluorescence study of DNA helicase activity

T. Ha, H. P. Babcock, W. Cheng, T. M. Lohman, S. Chu

Research output: Contribution to conference › Paper › peer-review

Abstract

The molecular mechanism of helicases at the single molecule level was studied using fluorescence resonance energy transfer (FRET) between two dyes, donor and acceptor. Measured FRET efficiency was shown to be an indicator of the distance between two dyes, revealing the extent and the time-course of unwinding.

Original language	English
Pages	589-590
Number of pages	2
DOIs	https://doi.org/10.1109/cleo.2000.907430
State	Published - Jan 1 2000
Event	Conference on Lasers and Electro-Optics (CLEO 2000) - San Francisco, CA, USA Duration: May 7 2000 → May 12 2000

Conference

Conference	Conference on Lasers and Electro-Optics (CLEO 2000)
City	San Francisco, CA, USA
Period	05/7/00 → 05/12/00

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10.1109/cleo.2000.907430

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title = "Single molecule fluorescence study of DNA helicase activity",

abstract = "The molecular mechanism of helicases at the single molecule level was studied using fluorescence resonance energy transfer (FRET) between two dyes, donor and acceptor. Measured FRET efficiency was shown to be an indicator of the distance between two dyes, revealing the extent and the time-course of unwinding.",

author = "T. Ha and Babcock, {H. P.} and W. Cheng and Lohman, {T. M.} and S. Chu",

year = "2000",

month = jan,

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doi = "10.1109/cleo.2000.907430",

language = "English",

pages = "589--590",

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T1 - Single molecule fluorescence study of DNA helicase activity

AU - Ha, T.

AU - Babcock, H. P.

AU - Cheng, W.

AU - Lohman, T. M.

AU - Chu, S.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - The molecular mechanism of helicases at the single molecule level was studied using fluorescence resonance energy transfer (FRET) between two dyes, donor and acceptor. Measured FRET efficiency was shown to be an indicator of the distance between two dyes, revealing the extent and the time-course of unwinding.

AB - The molecular mechanism of helicases at the single molecule level was studied using fluorescence resonance energy transfer (FRET) between two dyes, donor and acceptor. Measured FRET efficiency was shown to be an indicator of the distance between two dyes, revealing the extent and the time-course of unwinding.

UR - http://www.scopus.com/inward/record.url?scp=0034542189&partnerID=8YFLogxK

U2 - 10.1109/cleo.2000.907430

DO - 10.1109/cleo.2000.907430

M3 - Paper

AN - SCOPUS:0034542189

SP - 589

EP - 590

Y2 - 7 May 2000 through 12 May 2000

ER -

Single molecule fluorescence study of DNA helicase activity

Abstract

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