TY - JOUR
T1 - Single cell transcriptomics reveals opioid usage evokes widespread suppression of antiviral gene program
AU - Karagiannis, Tanya T.
AU - Cleary, John P.
AU - Gok, Busra
AU - Henderson, Andrew J.
AU - Martin, Nicholas G.
AU - Yajima, Masanao
AU - Nelson, Elliot C.
AU - Cheng, Christine S.
N1 - Funding Information:
We thank Dr. Todd A. Blute for his technical support on flow cytometry analysis. We would like to thank Dr. David J. Waxman for critical reading of the manuscript. The work was supported by NIH (R61DA047032 to C.S.C.). C.S.C. was also supported by Boston University Data Science Faculty Fellowship. T.T.K was also supported by NIH institutional training grant T32GM100842. CATS data collection was funded by R01DA17305 and E.C.N. is supported by R01DA042620, R01DA046436, and R33DA041883. A.J.H. is supported by NIH R61DA047032.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Chronic opioid usage not only causes addiction behavior through the central nervous system, but also modulates the peripheral immune system. However, how opioid impacts the immune system is still barely characterized systematically. In order to understand the immune modulatory effect of opioids in an unbiased way, here we perform single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells from opioid-dependent individuals and controls to show that chronic opioid usage evokes widespread suppression of antiviral gene program in naive monocytes, as well as in multiple immune cell types upon stimulation with the pathogen component lipopolysaccharide. Furthermore, scRNA-seq reveals the same phenomenon after a short in vitro morphine treatment. These findings indicate that both acute and chronic opioid exposure may be harmful to our immune system by suppressing the antiviral gene program. Our results suggest that further characterization of the immune modulatory effects of opioid is critical to ensure the safety of clinical opioids.
AB - Chronic opioid usage not only causes addiction behavior through the central nervous system, but also modulates the peripheral immune system. However, how opioid impacts the immune system is still barely characterized systematically. In order to understand the immune modulatory effect of opioids in an unbiased way, here we perform single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells from opioid-dependent individuals and controls to show that chronic opioid usage evokes widespread suppression of antiviral gene program in naive monocytes, as well as in multiple immune cell types upon stimulation with the pathogen component lipopolysaccharide. Furthermore, scRNA-seq reveals the same phenomenon after a short in vitro morphine treatment. These findings indicate that both acute and chronic opioid exposure may be harmful to our immune system by suppressing the antiviral gene program. Our results suggest that further characterization of the immune modulatory effects of opioid is critical to ensure the safety of clinical opioids.
UR - http://www.scopus.com/inward/record.url?scp=85085514050&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-16159-y
DO - 10.1038/s41467-020-16159-y
M3 - Article
C2 - 32457298
AN - SCOPUS:85085514050
SN - 2041-1723
VL - 11
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 2611
ER -