Single-cell RNA sequencing reveals the induction of novel myeloid and myeloid-associated cell populations in visceral fat with long-term obesity

Natalia S. Harasymowicz, Neda Rashidi, Alireza Savadipour, Chia Lung Wu, Ruhang Tang, John Bramley, William Buchser, Farshid Guilak

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Macrophages and other immune cells are important contributors to obesity-associated inflammation; however, the cellular identities of these specific populations remain unknown. In this study, we identified individual populations of myeloid cells found in mouse epididymal/visceral adipose tissue by single-cell RNA sequencing, immunofluorescence, and flow cytometry. Multiple canonical correlation analysis identified 11 unique myeloid and myeloid-associate cell populations. In obese mice, we detected an increased percentage of monocyte-derived pro-inflammatory cells expressing Cd9 and Trem2, as well as significantly decreased percentages of multiple cell populations, including tissue-resident cells expressing Lyve1, Mafb, and Mrc1. We have identified and validated a novel myeloid/macrophage population defined by Ly6a expression, exhibiting both myeloid and mesenchymal characteristics, which increased with obesity and showed high pro-fibrotic characteristics in vitro. Our mouse adipose tissue myeloid cell atlas provides an important resource to investigate obesity-associated inflammation and fibrosis.

Original languageEnglish
Article numbere21417
JournalFASEB Journal
Volume35
Issue number3
DOIs
StatePublished - Mar 2021

Keywords

  • adipokine
  • canonical correlation analysis
  • collagen
  • fibrotic
  • metabolic syndrome
  • scRNA-seq

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