TY - GEN
T1 - Single cell per well trapping and analysis of chlamydomonas reinhardtii using surface acoustic waves
AU - Cui, Mingyang
AU - Bayly, Philip V.
AU - Dutcher, Susan K.
AU - Meacham, J. Mark
N1 - Funding Information:
This work was supported by the National Science Foundation, under Grant No. CMMI-1633971. Fabrication of glass SAW devices was conducted in the Institute of Materials Science and Engineering (IMSE) cleanroom at Washington University in St. Louis. The authors thank Dr. Mathieu Bottier for supporting cell preparation.
Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - Acoustic manipulation shows significant promise for label-free and non-contact control and analysis of microorganisms in biology and biomedicine. However, trapping of highly-motile microswimmers like the single-cell algae Chlamydomonas reinhardtii (CR) is challenging because generation of sufficient trapping force can heat cells beyond their thermotolerance threshold. Herein, we introduce a surface acoustic wave (SAW)-driven device incorporating a glass microchamber, which enables trapping of individual CR cells without excessive heating. Trapped cells were imaged with high spatial and temporal resolution for analysis of the oscillatory motion of propulsive cilia. Ciliary frequency, waveform, curvature, and amplitude are compared for biciliated and uniciliated cells.
AB - Acoustic manipulation shows significant promise for label-free and non-contact control and analysis of microorganisms in biology and biomedicine. However, trapping of highly-motile microswimmers like the single-cell algae Chlamydomonas reinhardtii (CR) is challenging because generation of sufficient trapping force can heat cells beyond their thermotolerance threshold. Herein, we introduce a surface acoustic wave (SAW)-driven device incorporating a glass microchamber, which enables trapping of individual CR cells without excessive heating. Trapped cells were imaged with high spatial and temporal resolution for analysis of the oscillatory motion of propulsive cilia. Ciliary frequency, waveform, curvature, and amplitude are compared for biciliated and uniciliated cells.
KW - Acoustic Trapping
KW - Ciliary Waveform Analysis
KW - Microswimmers
KW - Surface Acoustic Waves
UR - http://www.scopus.com/inward/record.url?scp=85098266037&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098266037
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 749
EP - 750
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -