@inproceedings{5b088161c3c845abba9f737b927507ef,
title = "Single cell per well trapping and analysis of chlamydomonas reinhardtii using surface acoustic waves",
abstract = "Acoustic manipulation shows significant promise for label-free and non-contact control and analysis of microorganisms in biology and biomedicine. However, trapping of highly-motile microswimmers like the single-cell algae Chlamydomonas reinhardtii (CR) is challenging because generation of sufficient trapping force can heat cells beyond their thermotolerance threshold. Herein, we introduce a surface acoustic wave (SAW)-driven device incorporating a glass microchamber, which enables trapping of individual CR cells without excessive heating. Trapped cells were imaged with high spatial and temporal resolution for analysis of the oscillatory motion of propulsive cilia. Ciliary frequency, waveform, curvature, and amplitude are compared for biciliated and uniciliated cells.",
keywords = "Acoustic Trapping, Ciliary Waveform Analysis, Microswimmers, Surface Acoustic Waves",
author = "Mingyang Cui and Bayly, {Philip V.} and Dutcher, {Susan K.} and Meacham, {J. Mark}",
note = "Funding Information: This work was supported by the National Science Foundation, under Grant No. CMMI-1633971. Fabrication of glass SAW devices was conducted in the Institute of Materials Science and Engineering (IMSE) cleanroom at Washington University in St. Louis. The authors thank Dr. Mathieu Bottier for supporting cell preparation. Publisher Copyright: {\textcopyright} 2020 CBMS-0001; null ; Conference date: 04-10-2020 Through 09-10-2020",
year = "2020",
language = "English",
series = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "749--750",
booktitle = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
}