Single cell per well trapping and analysis of chlamydomonas reinhardtii using surface acoustic waves

Mingyang Cui, Philip V. Bayly, Susan K. Dutcher, J. Mark Meacham

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Acoustic manipulation shows significant promise for label-free and non-contact control and analysis of microorganisms in biology and biomedicine. However, trapping of highly-motile microswimmers like the single-cell algae Chlamydomonas reinhardtii (CR) is challenging because generation of sufficient trapping force can heat cells beyond their thermotolerance threshold. Herein, we introduce a surface acoustic wave (SAW)-driven device incorporating a glass microchamber, which enables trapping of individual CR cells without excessive heating. Trapped cells were imaged with high spatial and temporal resolution for analysis of the oscillatory motion of propulsive cilia. Ciliary frequency, waveform, curvature, and amplitude are compared for biciliated and uniciliated cells.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages749-750
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: Oct 4 2020Oct 9 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period10/4/2010/9/20

Keywords

  • Acoustic Trapping
  • Ciliary Waveform Analysis
  • Microswimmers
  • Surface Acoustic Waves

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