Single-cell Motility Analysis of Tethered Human Spermatozoa

William M. Skinner; Nadja Mannowetz; Polina V. Lishko; Nadia R. Roan

doi:10.21769/BioProtoc.3182

Single-cell Motility Analysis of Tethered Human Spermatozoa

William M. Skinner, Nadja Mannowetz, Polina V. Lishko, Nadia R. Roan

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Vigorous sperm flagellar motility is essential for fertilization, and so the quantitative measurement of motility is a useful tool to assess the intrinsic fertility potential of sperm cells and explore how various factors can alter sperm’s ability to reach the egg and penetrate its protective layers. Human sperm beat their flagella many times each second, and so recording and accurately quantifying this movement requires a high-speed camera. The aim of this protocol is to provide a detailed description of the tools required for quantitative beat frequency measurement of tethered human sperm at the single-cell level and to describe methods for investigating the effects of intracellular or extracellular factors on flagellar motion. This assay complements bulk measurements of sperm parameters using commercially-available systems for computer-assisted sperm analysis (CASA).

Original language	English
Article number	e3182
Journal	Bio-protocol
Volume	9
Issue number	5
DOIs	https://doi.org/10.21769/BioProtoc.3182
State	Published - Mar 5 2019

Keywords

Amyloid fibrils
Flagellar beat frequency
High-speed imaging
Human spermatozoa
Seminal plasma
Single-cell motility analysis
Sperm Swim-up
Sperm flagellum
Sperm motility

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10.21769/BioProtoc.3182

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title = "Single-cell Motility Analysis of Tethered Human Spermatozoa",

abstract = "Vigorous sperm flagellar motility is essential for fertilization, and so the quantitative measurement of motility is a useful tool to assess the intrinsic fertility potential of sperm cells and explore how various factors can alter sperm{\textquoteright}s ability to reach the egg and penetrate its protective layers. Human sperm beat their flagella many times each second, and so recording and accurately quantifying this movement requires a high-speed camera. The aim of this protocol is to provide a detailed description of the tools required for quantitative beat frequency measurement of tethered human sperm at the single-cell level and to describe methods for investigating the effects of intracellular or extracellular factors on flagellar motion. This assay complements bulk measurements of sperm parameters using commercially-available systems for computer-assisted sperm analysis (CASA).",

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AU - Skinner, William M.

AU - Mannowetz, Nadja

AU - Lishko, Polina V.

AU - Roan, Nadia R.

N1 - Publisher Copyright: Copyright Skinner et al.

PY - 2019/3/5

Y1 - 2019/3/5

N2 - Vigorous sperm flagellar motility is essential for fertilization, and so the quantitative measurement of motility is a useful tool to assess the intrinsic fertility potential of sperm cells and explore how various factors can alter sperm’s ability to reach the egg and penetrate its protective layers. Human sperm beat their flagella many times each second, and so recording and accurately quantifying this movement requires a high-speed camera. The aim of this protocol is to provide a detailed description of the tools required for quantitative beat frequency measurement of tethered human sperm at the single-cell level and to describe methods for investigating the effects of intracellular or extracellular factors on flagellar motion. This assay complements bulk measurements of sperm parameters using commercially-available systems for computer-assisted sperm analysis (CASA).

AB - Vigorous sperm flagellar motility is essential for fertilization, and so the quantitative measurement of motility is a useful tool to assess the intrinsic fertility potential of sperm cells and explore how various factors can alter sperm’s ability to reach the egg and penetrate its protective layers. Human sperm beat their flagella many times each second, and so recording and accurately quantifying this movement requires a high-speed camera. The aim of this protocol is to provide a detailed description of the tools required for quantitative beat frequency measurement of tethered human sperm at the single-cell level and to describe methods for investigating the effects of intracellular or extracellular factors on flagellar motion. This assay complements bulk measurements of sperm parameters using commercially-available systems for computer-assisted sperm analysis (CASA).

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KW - Flagellar beat frequency

KW - High-speed imaging

KW - Human spermatozoa

KW - Seminal plasma

KW - Single-cell motility analysis

KW - Sperm Swim-up

KW - Sperm flagellum

KW - Sperm motility

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Single-cell Motility Analysis of Tethered Human Spermatozoa

Abstract

Keywords

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