Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids

Keyphrases

• Gene Knockout 100%
• Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) 100%
• Guide RNA (gRNA) 100%
• Concatemer 100%
• Small Intestinal Organoids 100%
• Organoids 75%
• CRISPR Technology 50%
• Wnt Signaling Pathway 25%
• Gene Editing 25%
• Loss Function 25%
• Genetic Modification 25%
• Gene Function 25%
• Biological Processes 25%
• Phenotypic Change 25%
• Negative Regulator 25%
• Stem Cell-derived 25%
• Double Knockout 25%
• Adult Stem Cells 25%
• Multiple Genes 25%
• Costing Methods 25%
• Expression Cassette 25%
• Simultaneous Delivery 25%
• Oligo 25%
• Paralogous Genes 25%
• Function Approach 25%
• Annealing 25%
• Synthetic DNA 25%
• R-spondin 25%
• Rapid Assembly 25%
• Gene Correction 25%
• Simultaneous Targeting 25%
• Genetic Compensation 25%

Biochemistry, Genetics and Molecular Biology

• Gene Targeting 100%
• Gene Knockout 100%
• CRISPR 100%
• Concatemer 100%
• Guide RNA 66%
• Enhancer Region 33%
• Wnt Signaling Pathway 33%
• Genetics 33%
• Gene Knockin 33%
• Genome Editing 33%
• Biological Phenomena and Functions Concerning the Entire Organism 33%
• Gene Function 33%
• DNA Modification 33%
• Adult Stem Cell 33%
• Expression Cassette 33%

Neuroscience

• CRISPR 100%
• Concatemer 100%
• Organoid 100%
• Gene Knockout 60%
• Guide RNA 40%
• Stem Cell 20%
• Enhancer Region 20%
• Wnt Signaling Pathway 20%
• Gene Function 20%
• Synthetic DNA 20%
• Gene Knockin 20%