Simultaneous eye tracking and single-neuron recordings in human epilepsy patients

Shuo Wang, Nand Chandravadia, Adam N. Mamelak, Ueli Rutishauser

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Intracranial recordings from patients with intractable epilepsy provide a unique opportunity to study the activity of individual human neurons during active behavior. An important tool for quantifying behavior is eye tracking, which is an indispensable tool for studying visual attention. However, eye tracking is challenging to use concurrently with invasive electrophysiology and this approach has consequently been little used. Here, we present a proven experimental protocol to conduct single-neuron recordings with simultaneous eye tracking in humans. We describe how the systems are connected and the optimal settings to record neurons and eye movements. To illustrate the utility of this method, we summarize results that were made possible by this setup. This data shows how using eye tracking in a memory-guided visual search task allowed us to describe a new class of neurons called target neurons, whose response was reflective of top-down attention to the current search target. Lastly, we discuss the significance and solutions to potential problems of this setup. Together, our protocol and results suggest that single-neuron recordings with simultaneous eye tracking in humans are an effective method to study human brain function. It provides a key missing link between animal neurophysiology and human cognitive neuroscience.

Original languageEnglish
Article numbere59117
JournalJournal of Visualized Experiments
Issue number148
StatePublished - Jun 2019


  • Attention
  • Behavior
  • Epilepsy patients
  • Eye tracking
  • Human single-neuron recording
  • Issue 148
  • Medial temporal lobe
  • Target detection
  • Visual search


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