Simultaneous ex vivo functional testing of two retinas by in vivo electroretinogram system

Frans Vinberg, Vladimir Kefalov

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

An In vivo electroretinogram (ERG) signal is composed of several overlapping components originating from different retinal cell types, as well as noise from extra-retinal sources. Ex vivo ERG provides an efficient method to dissect the function of retinal cells directly from an intact isolated retina of animals or donor eyes. In addition, ex vivo ERG can be used to test the efficacy and safety of potential therapeutic agents on retina tissue from animals or humans. We show here how commercially available in vivo ERG systems can be used to conduct ex vivo ERG recordings from isolated mouse retinas. We combine the light stimulation, electronic and heating units of a standard in vivo system with customdesigned specimen holder, gravity-controlled perfusion system and electromagnetic noise shielding to record low-noise ex vivo ERG signals simultaneously from two retinas with the acquisition software included in commercial in vivo systems. Further, we demonstrate how to use this method in combination with pharmacological treatments that remove specific ERG components in order to dissect the function of certain retinal cell types.

Original languageEnglish
Article numbere52855
JournalJournal of Visualized Experiments
Volume2015
Issue number99
DOIs
StatePublished - May 6 2015

Keywords

  • Cone
  • Drug testing
  • ERG
  • Electrophysiology
  • Electroretinogram
  • Issue 99
  • Neuroscience
  • Photoreceptor
  • Retina
  • Rod
  • a-wave
  • b-wave
  • ex vivo ERG

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