Simultaneous evaluation of dual gene transfer to adherent cells by gamma-ray imaging

Kurt R. Zinn, Tandra R. Chaudhuri, Donald J. Buchsbaum, James M. Mountz, Buck E. Rogers

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A gamma camera imaging method was developed to detect dual gene transfer to adherent cells growing as monolayers in cell culture plates. Human cancer cells were infected with replication-incompetent adenoviral vectors encoding the human type 2 somatostatin receptor (Ad-hSSTr2) and/or herpes virus thymidine kinase (Ad-TK). The hSSTr2 and TK reporter proteins were detected by imaging internally bound 99mTc-P2045 peptide (Diatide, Inc.) and radioiodinated 2′-deoxy-2′-fluoro-β-D-arabinofuranosyl-5-iodouracil (FIAU), respectively. Following gene transfer, expression of hSSTr2 and TK were accurately imaged in vitro.

Original languageEnglish
Pages (from-to)135-144
Number of pages10
JournalNuclear Medicine and Biology
Volume28
Issue number2
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Keywords

  • Gene transfer
  • I
  • Imaging
  • Somatostatin receptor
  • Tc
  • Thymidine kinase

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