Abstract
A gamma camera imaging method was developed to detect dual gene transfer to adherent cells growing as monolayers in cell culture plates. Human cancer cells were infected with replication-incompetent adenoviral vectors encoding the human type 2 somatostatin receptor (Ad-hSSTr2) and/or herpes virus thymidine kinase (Ad-TK). The hSSTr2 and TK reporter proteins were detected by imaging internally bound 99mTc-P2045 peptide (Diatide, Inc.) and radioiodinated 2′-deoxy-2′-fluoro-β-D-arabinofuranosyl-5-iodouracil (FIAU), respectively. Following gene transfer, expression of hSSTr2 and TK were accurately imaged in vitro.
Original language | English |
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Pages (from-to) | 135-144 |
Number of pages | 10 |
Journal | Nuclear Medicine and Biology |
Volume | 28 |
Issue number | 2 |
DOIs | |
State | Published - 2001 |
Keywords
- Gene transfer
- I
- Imaging
- Somatostatin receptor
- Tc
- Thymidine kinase