Silk-Encapsulated Plasmonic Biochips with Enhanced Thermal Stability

Congzhou Wang, Jingyi Luan, Sirimuvva Tadepalli, Keng Ku Liu, Jeremiah J. Morrissey, Evan D. Kharasch, Rajesh R. Naik, Srikanth Singamaneni

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Because of their high sensitivity, cost-efficiency, and great potential as point-of-care biodiagnostic devices, plasmonic biosensors based on localized surface plasmon resonance have gained immense attention. However, most plasmonic biosensors and conventional bioassays rely on natural antibodies, which are susceptible to elevated temperatures and nonaqueous media. Hence, an expensive and cumbersome "cold chain" system is necessary to preserve the labile antibodies by maintaining optimal cold temperatures during transport, storage, and handling. Herein, we introduce a facile approach to preserve the antibody activity on a biosensor surface even at elevated temperatures. We show that silk fibroin film could be used as a protective layer to preserve the activity of a model antibody (Rabbit IgG) and cardiac troponin antibody at both room temperature and 40 °C over several days. Furthermore, a simple aqueous rinsing process restores the biofunctionality of the biosensor. This energy-efficient and environmentally friendly method represents a novel approach to eliminate the cold chain and temperature-controlled packing of diagnostic reagents and materials, thereby extending the capability of antibody-based biosensors to different resource-limited circumstances such as developing countries, an ambulance, an intensive care unit emergency room, and battlefield.

Original languageEnglish
Pages (from-to)26493-26500
Number of pages8
JournalACS Applied Materials and Interfaces
Volume8
Issue number40
DOIs
StatePublished - Oct 12 2016

Keywords

  • biopreservation
  • gold nanorods
  • localized surface plasmon resonance
  • plasmonic biosensor
  • silk

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