TY - JOUR
T1 - Silence of the strands
T2 - RNA interference in eukaryotic pathogens
AU - Cottrell, Tricia R.
AU - Doering, Tamara L.
N1 - Funding Information:
For helpful discussions and communication of unpublished results we gratefully acknowledge members of the Doering lab, Mark Drew, Nina Agabian, Steve Beverley, Paul Englund, Bill Goldman, Lena Hwang, Kelly Robinson, David Roos, Sergio Schenkman, Glen Shearer, David Sibley, Anita Sil, Sam Stanley and Elisabetta Ullu. We thank Elisabetta Ullu for encouragement in pursuing this technique in fungi and Mark Drew, Bill Goldman and Naomi Morrissette for comments on the manuscript. Finally, we are grateful to Norma Andrews and Bill Goldman for fostering joint discussion of protozoan parasites and fungi at the 2002 Gordon Conference on the Biology of Host–Parasite Interactions. Work in our laboratory is supported by NIH AI49173 and GM66303, a Burroughs Wellcome Fund Junior Investigator Award in Molecular Pathogenic Mycology, and the Andrew and Virginia Craig Faculty Research Fund. T.R.C. was partly supported during this work by an Undergraduate Research Fellowship from the American Society of Microbiology.
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Double-stranded (ds) RNA interference (RNAi) is a recent technological advance that enables researchers to reduce gene expression at the post-transcriptional level. This form of RNA silencing is initiated by dsRNA, expressed in or introduced into a cell of interest, which triggers homology-dependent degradation of the corresponding mRNA. This versatile technique has remarkable promise as a tool for the study of eukaryotic pathogens. Protozoan parasites and pathogenic fungi often resist manipulation using standard molecular genetic approaches. Researchers studying these organisms need flexible molecular tools, particularly to exploit newly sequenced genomes; this review offers a practical guide to establishing RNAi in pathogenic eukaryotes.
AB - Double-stranded (ds) RNA interference (RNAi) is a recent technological advance that enables researchers to reduce gene expression at the post-transcriptional level. This form of RNA silencing is initiated by dsRNA, expressed in or introduced into a cell of interest, which triggers homology-dependent degradation of the corresponding mRNA. This versatile technique has remarkable promise as a tool for the study of eukaryotic pathogens. Protozoan parasites and pathogenic fungi often resist manipulation using standard molecular genetic approaches. Researchers studying these organisms need flexible molecular tools, particularly to exploit newly sequenced genomes; this review offers a practical guide to establishing RNAi in pathogenic eukaryotes.
UR - http://www.scopus.com/inward/record.url?scp=0037232973&partnerID=8YFLogxK
U2 - 10.1016/S0966-842X(02)00004-5
DO - 10.1016/S0966-842X(02)00004-5
M3 - Review article
C2 - 12526853
AN - SCOPUS:0037232973
SN - 0966-842X
VL - 11
SP - 37
EP - 43
JO - Trends in Microbiology
JF - Trends in Microbiology
IS - 1
ER -