Signaling through TNF receptor p55 in TNF-α-deficient mice alters the CXCL13/CCL19/CCL21 ratio in the spleen and induces maturation and migration of anergic B cells into the B cell follicle

L. Mandik-Nayak, G. Huang, K. C.F. Sheehan, J. Erikson, D. D. Chaplin

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The organization of secondary lymphoid tissues into distinct T and B cell compartments supports proper regulation of an immune response to foreign Ags. In the splenic white pulp, this compartmentalization is also thought to be important in the maintenance of B cell tolerance. Using lymphotoxin-α-(LT-α)-, TNF-α-, or TNFRp55-deficient mice, all with disrupted splenic architecture, we tested whether normal T/B segregation and/or intact follicular structure are necessary for the maintenance of anti-dsDNA B cell anergy. This study demonstrates that anti-dsDNA B cells remain tolerant in LT-α-/-, TNF-α-/-, and TNFRp55-/- mice; however, TNF-α or a TNF-α-dependent factor is required for their characteristic positioning to the T/B interface. Providing a TNF-α signal in TNF-α-/- mice by systemic administration of an agonist anti-TNFRp55 mAb induces the maturation of the anti-dsDNA B cells and their movement away from the T cell area toward the B cell area. Additionally, the agonist Ab induces changes in the follicular environment, including FDC clustering, up-regulation of the CXC chemokine ligand CXCL13, and down-regulation of the CC chemokine ligands CCL19 and CCL21. Therefore, this study suggests that a balance between B and T cell tropic chemokine signals may be an important mechanism for positioning anergic B cells at the T/B interface of the splenic white pulp.

Original languageEnglish
Pages (from-to)1920-1928
Number of pages9
JournalJournal of Immunology
Volume167
Issue number4
StatePublished - Aug 15 2001
Externally publishedYes

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