Signal characteristics of G protein-transactivated EGF receptor

Henrik Daub, Christian Wallasch, Andreas Lankenau, Andreas Herrlich, Axel Ullrich

Research output: Contribution to journalArticlepeer-review

584 Scopus citations

Abstract

The epidermal growth factor receptor (EGFR) tyrosine kinase recently was identified as providing a link to mitogen-activated protein kinase (MAPK) in response to G protein-coupled receptor (GPCR) agonists in Rat-1 fibroblasts. This cross-talk pathway is also established in other cell types such as HaCaT keratinocytes, primary mouse astrocytes and COS-7 cells. Transient expression of either G(q)- or G(i)-coupled receptors in COS-7 cells allowed GPCR agonist-induced EGFR transactivation, and lysophosphatidic acid (LPA)-generated signals involved the docking protein Gab1. The increase in SHC tyrosine phosphorylation and MAPK stimulation through both G(q)- and G(i)-coupled receptors was reduced strongly upon selective inhibition of EGFR function. Inhibition of phosphoinositide 3-kinase did not affect GPCR-induced stimulation of EGFR tyrosine phosphorylation, but inhibited MAPK stimulation, upon treatment with both GPCR agonists and low doses of EGF. Furthermore, the Src tyrosine kinase inhibitor PP1 strongly interfered with LPA- and EGF-induced tyrosine phosphorylation and MAPK activation downstream of EGFR. Our results demonstrate an essential role for EGFR function in signaling through both G(q)- and G(i)-coupled receptors and provide novel insights into signal transmission downstream of EGFR for efficient activation of the Ras/MAPK pathway.

Original languageEnglish
Pages (from-to)7032-7044
Number of pages13
JournalEMBO Journal
Volume16
Issue number23
DOIs
StatePublished - Dec 1 1997

Keywords

  • EGF receptor
  • G protein-coupled receptors
  • MAP kinase
  • PI3-kinase
  • Src

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