Shortening of poly (A) tail of glucose transporter-one mRNA in experimental diabetes mellitus

Gul N. Shah, Stephen J. Giddings, Arshag D. Mooradian

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12 Scopus citations

Abstract

To determine the molecular mechanisms of diabetes-related changes in the expression of GLUT-1 in cerebral tissue, streptozotocin-induced diabetic rats and vehicle injected controls were studied after 4 weeks of diabetes. The GLUT-1 mass in cerebral microvessels was reduced in diabetic rats by approximately 38% (P < 0.01). The GLUT-1 concentration in insulin-treated diabetic group was not significantly different from controls. The GLUT-1 mRNA content of cerebral tissue in diabetic rats (0.064 ± 0.007) was significantly reduced compared to control rats (0.122 ± 0.011) or insulin-treated diabetic rats (0.122 ± 0.015) P < 0.01. The in vitro translation of GLUT-1 mRNA of diabetic rats (0.793 ± 0.047 arbitrary units) was also significantly lower than that in control rats (1.403 ± 0.153) P < 0.01 or insulin-treated diabetic rats. (1.124 ± 0.083) P < 0.01. These changes occurred in asssociation with a reduction in poly (A) tail length of GLUT-1 mRNA which decreased from a control value of 200-350 nt to only 50-100 nt in diabetic rats. Shortening of poly (A) tail of mRNAs is a novel mechanism of diabetes-related changes in the expression of specific genes which are regulated at a translational level.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalBrain Research
Volume754
Issue number1-2
DOIs
StatePublished - Apr 18 1997

Keywords

  • blood-brain barrier
  • diabetes
  • glucose transport
  • poly (A) tail

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