Short communication: Vascular smooth muscle cell stiffness as a mechanism for increased aortic stiffness with aging

Hongyu Qiu, Yi Zhu, Zhe Sun, Jerome P. Trzeciakowski, Meredith Gansner, Christophe Depre, Ranillo R.G. Resuello, Filipinas F. Natividad, William C. Hunter, Guy M. Genin, Elliot L. Elson, Dorothy E. Vatner, Gerald A. Meininger, Stephen F. Vatner

Research output: Contribution to journalArticle

180 Scopus citations

Abstract

Rationale: Increased aortic stiffness, an important feature of many vascular diseases, eg, aging, hypertension, atherosclerosis, and aortic aneurysms, is assumed because of changes in extracellular matrix (ECM). Objective: We tested the hypothesis that the mechanisms also involve intrinsic stiffening of vascular smooth muscle cells (VSMCs). Methods and Results: Stiffness was measured in vitro both by atomic force microscopy (AFM) and in a reconstituted tissue model, using VSMCs from aorta of young versus old male monkeys (Macaca fascicularis) (n=7/group), where aortic stiffness increases by 200% in vivo. The apparent elastic modulus was increased (P<0.05) in old (41.7±0.5 kPa) versus young (12.8±0.3 kPa) VSMCs but not after disassembly of the actin cytoskeleton with cytochalasin D. Stiffness of the VSMCs in the reconstituted tissue model was also higher (P<0.05) in old (23.3±3.0 kPa) than in young (13.7±2.4 kPa). Conclusions: These data support the novel concept, not appreciated previously, that increased vascular stiffness with aging is attributable not only to changes in ECM but also to intrinsic changes in VSMCs.

Original languageEnglish
Pages (from-to)615-619
Number of pages5
JournalCirculation research
Volume107
Issue number5
DOIs
StatePublished - Sep 3 2010

Keywords

  • AFM
  • aging
  • stiffness
  • vascular smooth muscle cell

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    Qiu, H., Zhu, Y., Sun, Z., Trzeciakowski, J. P., Gansner, M., Depre, C., Resuello, R. R. G., Natividad, F. F., Hunter, W. C., Genin, G. M., Elson, E. L., Vatner, D. E., Meininger, G. A., & Vatner, S. F. (2010). Short communication: Vascular smooth muscle cell stiffness as a mechanism for increased aortic stiffness with aging. Circulation research, 107(5), 615-619. https://doi.org/10.1161/CIRCRESAHA.110.221846