TY - JOUR
T1 - Shiga toxin-producing Escherichia coli in Montana
T2 - Bacterial genotypes and clinical profiles
AU - Jelacic, Jill K.
AU - Damrow, Todd
AU - Chen, Gilbert S.
AU - Jelacic, Srdjan
AU - Bielaszewska, Martina
AU - Ciol, Marcia
AU - Carvalho, Humberto M.
AU - Melton-Celsa, Angela R.
AU - O'Brien, Alison D.
AU - Tarr, Phillip I.
N1 - Funding Information:
Financial support: Centers for Disease Control and Prevention (cooperative agreement U50/CCU814408, to fund laboratory surveillance for non-O157:H7 Shiga toxin–producing Escherichia coli); National Institutes of Health (grants AI-47499, to fund analysis of the isolates, and AI-20148, to fund work on stx2d-activatable); Bundesministerium für Bildung und Forschung Verbundprojekt (Forschungsnetzwerk 01KI9903).
PY - 2003/9/1
Y1 - 2003/9/1
N2 - The diseases and virulence genes associated with Shiga toxin-producing Escherichia coli (STEC) are characterized incompletely. We analyzed, by polymerase chain reaction, 82 STEC isolates collected prospectively in Montana and profiled associated illnesses by patient chart review. All E. coli O157:H7 contained stx2-group genes, as well as eae, iha, espA, and ehxA; 84% contained stx1. Non-O157:H7 STEC less frequently contained stx 1 (P = .046), stx2 (P < .001), iha (P < .001), eae, and espA (P = .039 for both), were isolated less often from patients treated in emergency departments (P = .022), and tended to be associated less frequently with bloody diarrhea (P = .061). There were no significant associations between stx genotype and bloody diarrhea, but isolates containing stx2c or stx2d-activatable were recovered more often from patients who underwent diagnostic or therapeutic procedures (P = .033). Non-O157:H7 STEC are more heterogeneous and cause bloody diarrhea less frequently than do E. coli O157:H7. Bloody diarrhea cannot be attributed simply to the stx genotype of the infecting organism.
AB - The diseases and virulence genes associated with Shiga toxin-producing Escherichia coli (STEC) are characterized incompletely. We analyzed, by polymerase chain reaction, 82 STEC isolates collected prospectively in Montana and profiled associated illnesses by patient chart review. All E. coli O157:H7 contained stx2-group genes, as well as eae, iha, espA, and ehxA; 84% contained stx1. Non-O157:H7 STEC less frequently contained stx 1 (P = .046), stx2 (P < .001), iha (P < .001), eae, and espA (P = .039 for both), were isolated less often from patients treated in emergency departments (P = .022), and tended to be associated less frequently with bloody diarrhea (P = .061). There were no significant associations between stx genotype and bloody diarrhea, but isolates containing stx2c or stx2d-activatable were recovered more often from patients who underwent diagnostic or therapeutic procedures (P = .033). Non-O157:H7 STEC are more heterogeneous and cause bloody diarrhea less frequently than do E. coli O157:H7. Bloody diarrhea cannot be attributed simply to the stx genotype of the infecting organism.
UR - http://www.scopus.com/inward/record.url?scp=0141501143&partnerID=8YFLogxK
U2 - 10.1086/376999
DO - 10.1086/376999
M3 - Article
C2 - 12934188
AN - SCOPUS:0141501143
SN - 0022-1899
VL - 188
SP - 719
EP - 729
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -