Sexual differentiation of rat liver carbonic anhydrase III

Alan Shiels; Stephen Jeffery; Ian R. Phillips; Elizabeth A. Shephard; Catherine A. Wilson; Nicholas D. Carter

doi:10.1016/0304-4165(83)90370-7

Sexual differentiation of rat liver carbonic anhydrase III

Alan Shiels, Stephen Jeffery, Ian R. Phillips, Elizabeth A. Shephard, Catherine A. Wilson, Nicholas D. Carter

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Abstract

Using radioimmunoassay, the concentration of carbonic anhydrase III in the livers of adult male rats was found to be approx. 30-times greater than that observed in mature females. Castration of male rats led to a marked reduction in liver carbonic anhydrase III concentrations which could be partially restored to control levels by testosterone replacement. Administration of testosterone to ovariectomised female rats induced about a 5-fold increase in liver carbonic anhydrase III concentration. Immunoprecipitation analysis of the products of liver mRNA translation in vitro with antiserum specific for carbonic anhydrase III showed that hormonal control of the levels of carbonic anhydrase III in liver is mediated by changes in the amount of translatable carbonic anhydrase III mRNA. Marked changes in liver carbonic anhydrase III concentrations were also observed in developing and ageing male rats.

Original language	English
Pages (from-to)	335-342
Number of pages	8
Journal	BBA - General Subjects
Volume	760
Issue number	3
DOIs	https://doi.org/10.1016/0304-4165(83)90370-7
State	Published - Nov 8 1983

Keywords

(Rat liver)
Aging
Carbonic anhydrase
Oestradiol
Testosterone
mRNA

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10.1016/0304-4165(83)90370-7

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title = "Sexual differentiation of rat liver carbonic anhydrase III",

abstract = "Using radioimmunoassay, the concentration of carbonic anhydrase III in the livers of adult male rats was found to be approx. 30-times greater than that observed in mature females. Castration of male rats led to a marked reduction in liver carbonic anhydrase III concentrations which could be partially restored to control levels by testosterone replacement. Administration of testosterone to ovariectomised female rats induced about a 5-fold increase in liver carbonic anhydrase III concentration. Immunoprecipitation analysis of the products of liver mRNA translation in vitro with antiserum specific for carbonic anhydrase III showed that hormonal control of the levels of carbonic anhydrase III in liver is mediated by changes in the amount of translatable carbonic anhydrase III mRNA. Marked changes in liver carbonic anhydrase III concentrations were also observed in developing and ageing male rats.",

keywords = "(Rat liver), Aging, Carbonic anhydrase, Oestradiol, Testosterone, mRNA",

author = "Alan Shiels and Stephen Jeffery and Phillips, {Ian R.} and Shephard, {Elizabeth A.} and Wilson, {Catherine A.} and Carter, {Nicholas D.}",

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T1 - Sexual differentiation of rat liver carbonic anhydrase III

AU - Shiels, Alan

AU - Jeffery, Stephen

AU - Phillips, Ian R.

AU - Shephard, Elizabeth A.

AU - Wilson, Catherine A.

AU - Carter, Nicholas D.

PY - 1983/11/8

Y1 - 1983/11/8

N2 - Using radioimmunoassay, the concentration of carbonic anhydrase III in the livers of adult male rats was found to be approx. 30-times greater than that observed in mature females. Castration of male rats led to a marked reduction in liver carbonic anhydrase III concentrations which could be partially restored to control levels by testosterone replacement. Administration of testosterone to ovariectomised female rats induced about a 5-fold increase in liver carbonic anhydrase III concentration. Immunoprecipitation analysis of the products of liver mRNA translation in vitro with antiserum specific for carbonic anhydrase III showed that hormonal control of the levels of carbonic anhydrase III in liver is mediated by changes in the amount of translatable carbonic anhydrase III mRNA. Marked changes in liver carbonic anhydrase III concentrations were also observed in developing and ageing male rats.

AB - Using radioimmunoassay, the concentration of carbonic anhydrase III in the livers of adult male rats was found to be approx. 30-times greater than that observed in mature females. Castration of male rats led to a marked reduction in liver carbonic anhydrase III concentrations which could be partially restored to control levels by testosterone replacement. Administration of testosterone to ovariectomised female rats induced about a 5-fold increase in liver carbonic anhydrase III concentration. Immunoprecipitation analysis of the products of liver mRNA translation in vitro with antiserum specific for carbonic anhydrase III showed that hormonal control of the levels of carbonic anhydrase III in liver is mediated by changes in the amount of translatable carbonic anhydrase III mRNA. Marked changes in liver carbonic anhydrase III concentrations were also observed in developing and ageing male rats.

KW - (Rat liver)

KW - Aging

KW - Carbonic anhydrase

KW - Oestradiol

KW - Testosterone

KW - mRNA

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Sexual differentiation of rat liver carbonic anhydrase III

Abstract

Keywords

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