TY - JOUR
T1 - Serum markers may distinguish biliary atresia from other forms of neonatal cholestasis
AU - Wang, Hongtao
AU - Malone, James P.
AU - Gilmore, Petra Erdmann
AU - Davis, Alan E.
AU - Magee, John C.
AU - Townsend, R. Reid
AU - Heuckeroth, Robert O.
PY - 2010/4
Y1 - 2010/4
N2 - Background: Biliary atresia (BA) is the most serious liver disease in infants. Diagnosis currently depends on surgical exploration of the biliary tree. Noninvasive tests that distinguish BA from other types of neonatal liver disease are not available. Patients and Methods: To identify potential serum biomarkers that classify children with neonatal cholestasis, we performed 2-dimensional difference gel electrophoresis, statistical analysis, and tandem mass spectrometry using serum samples from 19 infants with BA and 19 infants with non-BA neonatal cholestasis. Results: Eleven potential serum biomarkers were found that could in combination classify children with neonatal cholestasis. Conclusions: Although no single biomarker or imaging test adequately distinguishes BA from other types of neonatal cholestasis, combinations of biomarkers, imaging tests, and noninvasive clinical criteria should be further explored as potential tests for rapid and accurate diagnosis of BA.
AB - Background: Biliary atresia (BA) is the most serious liver disease in infants. Diagnosis currently depends on surgical exploration of the biliary tree. Noninvasive tests that distinguish BA from other types of neonatal liver disease are not available. Patients and Methods: To identify potential serum biomarkers that classify children with neonatal cholestasis, we performed 2-dimensional difference gel electrophoresis, statistical analysis, and tandem mass spectrometry using serum samples from 19 infants with BA and 19 infants with non-BA neonatal cholestasis. Results: Eleven potential serum biomarkers were found that could in combination classify children with neonatal cholestasis. Conclusions: Although no single biomarker or imaging test adequately distinguishes BA from other types of neonatal cholestasis, combinations of biomarkers, imaging tests, and noninvasive clinical criteria should be further explored as potential tests for rapid and accurate diagnosis of BA.
KW - Biliary atresia
KW - Biomarker
KW - Neonatal cholestasis
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=77950235651&partnerID=8YFLogxK
U2 - 10.1097/MPG.0b013e3181cb42ee
DO - 10.1097/MPG.0b013e3181cb42ee
M3 - Article
C2 - 20216099
AN - SCOPUS:77950235651
VL - 50
SP - 411
EP - 416
JO - Journal of Pediatric Gastroenterology and Nutrition
JF - Journal of Pediatric Gastroenterology and Nutrition
SN - 0277-2116
IS - 4
ER -