@article{6e52c6848a1343d29bda827130f8e0ee,
title = "SEQUIN: An imaging and analysis platform for quantification and characterization of synaptic structures in mouse",
abstract = "Synapses are crucial to brain function and frequent disease targets, but current analysis methods cannot report on individual synaptic components in situ or present barriers to widespread adoption. SEQUIN was developed to address this challenge. SEQUIN utilizes a widely available super-resolution platform in tandem with image processing and analysis to quantify synaptic loci over large regions of brain and characterize their molecular and nanostructural properties at the individual and population level. This protocol describes quantification of synaptic loci using SEQUIN. For additional details on the use and execution of this protocol, please refer to Sauerbeck et al. (2020).",
keywords = "Microscopy, Neuroscience",
author = "Reitz, {Sydney J.} and Sauerbeck, {Andrew D.} and Kummer, {Terrance T.}",
note = "Funding Information: The authors wish to thank Dr. James Fitzpatrick and Matthew Curtis for imaging discussions. This study was supported by the BrightFocus and Brain Research Foundation, the McDonnell Center for Cellular and Molecular Neurobiology, and NIH I01BX005204 (all to T.T.K.). Experiments were performed in part through the Washington University Center for Cellular Imaging supported by Washington University School of Medicine, The Children's Discovery Institute, St. Louis Children's Hospital (CDI-CORE-2015-505), the Foundation for Barnes-Jewish Hospital (3770), and the NIH Office of Research Infrastructure Programs (OD021629). S.R. collected the data for this report. S.R. wrote the manuscript with input from A.D.S. and T.T.K. A.D.S. T.T.K. and S.R. developed the SEQUIN technique. T.T.K. was responsible for overall project management. The authors declare no competing interests. Funding Information: The authors wish to thank Dr. James Fitzpatrick and Matthew Curtis for imaging discussions. This study was supported by the BrightFocus and Brain Research Foundation , the McDonnell Center for Cellular and Molecular Neurobiology , and NIH I01BX005204 (all to T.T.K.). Experiments were performed in part through the Washington University Center for Cellular Imaging supported by Washington University School of Medicine , The Children{\textquoteright}s Discovery Institute , St. Louis Children{\textquoteright}s Hospital ( CDI-CORE-2015-505 ), the Foundation for Barnes-Jewish Hospital ( 3770 ), and the NIH Office of Research Infrastructure Programs ( OD021629 ). Publisher Copyright: {\textcopyright} 2021",
year = "2021",
month = mar,
day = "19",
doi = "10.1016/j.xpro.2020.100268",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
number = "1",
}