TY - JOUR
T1 - Sequential Study of the Histopathology and Cellular and Humoral Immune Response During the Development of an Autoimmune Orchitis in Wistar Rats
AU - DONCEL, GUSTAVO F.
AU - DI PAOLA, JORGE A.
AU - LUSTIG, LIVIA
PY - 1989/6
Y1 - 1989/6
N2 - ABSTRACT: Wistar rats immunized with an homologous testicular homogenate (TH) and complete Freund's adjuvant, followed by i.v. injection of Bordetella pertussis, developed an autoimmune orchitis (EAO). Animals were studied at 7, 16, 30, 50, and 80 days (d) after the first immunization. An important lesion of the testis only appeared at 50 d, increasing in severity and incidence (77%) at 80 d. Lesions were characterized by a prevalent aspermatogenesis with tubular atrophy and mild interstitial mononuclear infiltrates. Delayed‐type hypersensitivity response (DTH) against TH was detected early at 7 d and, except for 16 d, it increased with time, reaching a maximum at 80 d. A good temporal relationship between DTH and histopathology was found. Circulating antibodies to TH, detected by ELISA, were only present in 64% of the animals with testis lesion, while no deposits of IgG or C3 in the seminiferous tubules were seen. We describe a sequence of immunological events, concomitant with pathological changes of the testis, during the development of a severe EAO in Wistar rats. 1989 Munksgaard
AB - ABSTRACT: Wistar rats immunized with an homologous testicular homogenate (TH) and complete Freund's adjuvant, followed by i.v. injection of Bordetella pertussis, developed an autoimmune orchitis (EAO). Animals were studied at 7, 16, 30, 50, and 80 days (d) after the first immunization. An important lesion of the testis only appeared at 50 d, increasing in severity and incidence (77%) at 80 d. Lesions were characterized by a prevalent aspermatogenesis with tubular atrophy and mild interstitial mononuclear infiltrates. Delayed‐type hypersensitivity response (DTH) against TH was detected early at 7 d and, except for 16 d, it increased with time, reaching a maximum at 80 d. A good temporal relationship between DTH and histopathology was found. Circulating antibodies to TH, detected by ELISA, were only present in 64% of the animals with testis lesion, while no deposits of IgG or C3 in the seminiferous tubules were seen. We describe a sequence of immunological events, concomitant with pathological changes of the testis, during the development of a severe EAO in Wistar rats. 1989 Munksgaard
KW - Orchitis
KW - autoimmunity
KW - rat testis
UR - http://www.scopus.com/inward/record.url?scp=0024385252&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0897.1989.tb00638.x
DO - 10.1111/j.1600-0897.1989.tb00638.x
M3 - Article
C2 - 2803528
AN - SCOPUS:0024385252
SN - 1046-7408
VL - 20
SP - 44
EP - 51
JO - American Journal of Reproductive Immunology
JF - American Journal of Reproductive Immunology
IS - 2
ER -