TY - JOUR
T1 - Sequence of the human factor VIII-associated gene is conserved in mouse
AU - Levinson, Barbara
AU - Bermingham, John R.
AU - Metzenberg, Aida
AU - Kenwrick, Susan
AU - Chapman, Verne
AU - Gitschier, Jane
N1 - Funding Information:
We are grateful to Bruce Elder and Claude Nagamine for providing mouse tissues and blots, Bernadette Keitz for technical assistance, Fernando Bazan, Glenn Hammonds, and Russell Doolittle for helpful suggestions with the computer analysis, and Gail Herman and Cindy Faust for communicating unpublished physical mapping results in the mouse. This work was funded in part by grants from the NIH (HL42968 to J.G. and HG00277 to V.C.) _ J.G. is an assistant investigator with the Howard Hughes Medical Institute.
PY - 1992/7
Y1 - 1992/7
N2 - cDNA and genomic clones corresponding to the human factor VIII-associated gene (F8A) were isolated from mouse cDNA and F8A-enriched genomic libraries. The sequences of these clones revealed an intronless gene conding for 380 amino acids, with 85% identity the predicted human sequence. The single murine gene copy is genetically linked to factor VIII, but appears to lie outside the factor VIII gene by physical mapping. Like the human gene, the mouse F8A gene is highly expressed in a wide variety of tissues. This evolutionary comparison has helped to clarify the derived amino acid sequence in the human and strongly supports the hypothesis that the F8A gene encodes a protein.
AB - cDNA and genomic clones corresponding to the human factor VIII-associated gene (F8A) were isolated from mouse cDNA and F8A-enriched genomic libraries. The sequences of these clones revealed an intronless gene conding for 380 amino acids, with 85% identity the predicted human sequence. The single murine gene copy is genetically linked to factor VIII, but appears to lie outside the factor VIII gene by physical mapping. Like the human gene, the mouse F8A gene is highly expressed in a wide variety of tissues. This evolutionary comparison has helped to clarify the derived amino acid sequence in the human and strongly supports the hypothesis that the F8A gene encodes a protein.
UR - http://www.scopus.com/inward/record.url?scp=0026636824&partnerID=8YFLogxK
U2 - 10.1016/0888-7543(92)90170-W
DO - 10.1016/0888-7543(92)90170-W
M3 - Article
C2 - 1639415
AN - SCOPUS:0026636824
SN - 0888-7543
VL - 13
SP - 862
EP - 865
JO - Genomics
JF - Genomics
IS - 3
ER -