Sequence of the human factor VIII-associated gene is conserved in mouse

Barbara Levinson; John R. Bermingham; Aida Metzenberg; Susan Kenwrick; Verne Chapman; Jane Gitschier

doi:10.1016/0888-7543(92)90170-W

Sequence of the human factor VIII-associated gene is conserved in mouse

Barbara Levinson, John R. Bermingham, Aida Metzenberg, Susan Kenwrick, Verne Chapman, Jane Gitschier

Department of Genetics

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

cDNA and genomic clones corresponding to the human factor VIII-associated gene (F8A) were isolated from mouse cDNA and F8A-enriched genomic libraries. The sequences of these clones revealed an intronless gene conding for 380 amino acids, with 85% identity the predicted human sequence. The single murine gene copy is genetically linked to factor VIII, but appears to lie outside the factor VIII gene by physical mapping. Like the human gene, the mouse F8A gene is highly expressed in a wide variety of tissues. This evolutionary comparison has helped to clarify the derived amino acid sequence in the human and strongly supports the hypothesis that the F8A gene encodes a protein.

Original language	English
Pages (from-to)	862-865
Number of pages	4
Journal	Genomics
Volume	13
Issue number	3
DOIs	https://doi.org/10.1016/0888-7543(92)90170-W
State	Published - Jul 1992

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title = "Sequence of the human factor VIII-associated gene is conserved in mouse",

abstract = "cDNA and genomic clones corresponding to the human factor VIII-associated gene (F8A) were isolated from mouse cDNA and F8A-enriched genomic libraries. The sequences of these clones revealed an intronless gene conding for 380 amino acids, with 85% identity the predicted human sequence. The single murine gene copy is genetically linked to factor VIII, but appears to lie outside the factor VIII gene by physical mapping. Like the human gene, the mouse F8A gene is highly expressed in a wide variety of tissues. This evolutionary comparison has helped to clarify the derived amino acid sequence in the human and strongly supports the hypothesis that the F8A gene encodes a protein.",

author = "Barbara Levinson and Bermingham, {John R.} and Aida Metzenberg and Susan Kenwrick and Verne Chapman and Jane Gitschier",

note = "Funding Information: We are grateful to Bruce Elder and Claude Nagamine for providing mouse tissues and blots, Bernadette Keitz for technical assistance, Fernando Bazan, Glenn Hammonds, and Russell Doolittle for helpful suggestions with the computer analysis, and Gail Herman and Cindy Faust for communicating unpublished physical mapping results in the mouse. This work was funded in part by grants from the NIH (HL42968 to J.G. and HG00277 to V.C.) _ J.G. is an assistant investigator with the Howard Hughes Medical Institute.",

year = "1992",

month = jul,

doi = "10.1016/0888-7543(92)90170-W",

language = "English",

volume = "13",

pages = "862--865",

journal = "Genomics",

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T1 - Sequence of the human factor VIII-associated gene is conserved in mouse

AU - Levinson, Barbara

AU - Bermingham, John R.

AU - Metzenberg, Aida

AU - Kenwrick, Susan

AU - Chapman, Verne

AU - Gitschier, Jane

N1 - Funding Information: We are grateful to Bruce Elder and Claude Nagamine for providing mouse tissues and blots, Bernadette Keitz for technical assistance, Fernando Bazan, Glenn Hammonds, and Russell Doolittle for helpful suggestions with the computer analysis, and Gail Herman and Cindy Faust for communicating unpublished physical mapping results in the mouse. This work was funded in part by grants from the NIH (HL42968 to J.G. and HG00277 to V.C.) _ J.G. is an assistant investigator with the Howard Hughes Medical Institute.

PY - 1992/7

Y1 - 1992/7

N2 - cDNA and genomic clones corresponding to the human factor VIII-associated gene (F8A) were isolated from mouse cDNA and F8A-enriched genomic libraries. The sequences of these clones revealed an intronless gene conding for 380 amino acids, with 85% identity the predicted human sequence. The single murine gene copy is genetically linked to factor VIII, but appears to lie outside the factor VIII gene by physical mapping. Like the human gene, the mouse F8A gene is highly expressed in a wide variety of tissues. This evolutionary comparison has helped to clarify the derived amino acid sequence in the human and strongly supports the hypothesis that the F8A gene encodes a protein.

AB - cDNA and genomic clones corresponding to the human factor VIII-associated gene (F8A) were isolated from mouse cDNA and F8A-enriched genomic libraries. The sequences of these clones revealed an intronless gene conding for 380 amino acids, with 85% identity the predicted human sequence. The single murine gene copy is genetically linked to factor VIII, but appears to lie outside the factor VIII gene by physical mapping. Like the human gene, the mouse F8A gene is highly expressed in a wide variety of tissues. This evolutionary comparison has helped to clarify the derived amino acid sequence in the human and strongly supports the hypothesis that the F8A gene encodes a protein.

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U2 - 10.1016/0888-7543(92)90170-W

DO - 10.1016/0888-7543(92)90170-W

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JO - Genomics

JF - Genomics

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ER -

Sequence of the human factor VIII-associated gene is conserved in mouse

Abstract

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