TY - JOUR
T1 - Sequence heterogeneity of nef transcripts in HIV-1-infected subjects at different stages of disease
AU - Ratner, Lee
AU - Joseph, Theresa
AU - Bandres, Juan
AU - Ghosh, Sajal
AU - Vander Heyden, Nancy
AU - Templeton, Alan
AU - Hahn, Beatrice
AU - Powderly, William
AU - Arens, Max
N1 - Funding Information:
This work was supported by PHS Grants AI24745, 25903 and grants from AMFAR and the McDonnell Douglas Employees Community Fund.
PY - 1996/9/1
Y1 - 1996/9/1
N2 - Nef transcripts were analyzed from peripheral blood mononuclear cells of 10 HIV-1-infected subjects with 9-822 CD4+ lymphocytes/cu mm, including 4 individuals with a probable common source infection. There was no relationship between the phylogenetic position of the various net sequences and the disease state of the person from whom they were derived. The nef open reading frame was disrupted in all three clones from only 1 subject. Functional analyses of a representative clone from each of the remaining 9 subjects showed that all nef alleles were capable of CD4 cell surface down-regulation, but only three naf alleles suppressed the induction of IL-2 transcription.
AB - Nef transcripts were analyzed from peripheral blood mononuclear cells of 10 HIV-1-infected subjects with 9-822 CD4+ lymphocytes/cu mm, including 4 individuals with a probable common source infection. There was no relationship between the phylogenetic position of the various net sequences and the disease state of the person from whom they were derived. The nef open reading frame was disrupted in all three clones from only 1 subject. Functional analyses of a representative clone from each of the remaining 9 subjects showed that all nef alleles were capable of CD4 cell surface down-regulation, but only three naf alleles suppressed the induction of IL-2 transcription.
UR - http://www.scopus.com/inward/record.url?scp=0030246983&partnerID=8YFLogxK
U2 - 10.1006/viro.1996.0474
DO - 10.1006/viro.1996.0474
M3 - Article
C2 - 8806559
AN - SCOPUS:0030246983
SN - 0042-6822
VL - 223
SP - 245
EP - 250
JO - Virology
JF - Virology
IS - 1
ER -