Sequence heterogeneity of nef transcripts in HIV-1-infected subjects at different stages of disease

Lee Ratner, Theresa Joseph, Juan Bandres, Sajal Ghosh, Nancy Vander Heyden, Alan Templeton, Beatrice Hahn, William Powderly, Max Arens

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Nef transcripts were analyzed from peripheral blood mononuclear cells of 10 HIV-1-infected subjects with 9-822 CD4+ lymphocytes/cu mm, including 4 individuals with a probable common source infection. There was no relationship between the phylogenetic position of the various net sequences and the disease state of the person from whom they were derived. The nef open reading frame was disrupted in all three clones from only 1 subject. Functional analyses of a representative clone from each of the remaining 9 subjects showed that all nef alleles were capable of CD4 cell surface down-regulation, but only three naf alleles suppressed the induction of IL-2 transcription.

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalVirology
Volume223
Issue number1
DOIs
StatePublished - Sep 1 1996

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