Sequence elements required for apolipoprotein B mRNA editing enhancement activity from chicken enterocytes

Makoto Nakamuta, An Tsai, Lawrence Chan, Nicholas O. Davidson, Ba Bie Teng

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Mammalian intestinal apolipoprotein B (apoB) mRNA edits codon 2153 from CAA in apoB100 mRNA to a stop codon (UAA) in apoB48 mRNA. By contrast, chicken intestinal apoB mRNA contains a CAA codon at the corresponding site, but is not edited. Chicken enterocyte S100 extracts fail to edit mammalian apoB RNA, but contain factor(s) which enhance the mammalian enterocytes editing activity. By converting the chicken apoB mooring sequences to the conserved mammalian sequences, the study confirmed that this 11-nucleotide stretch was necessary and sufficient for minimal RNA editing. Using rat and chicken apoB chimeric constructs, the study revealed that mammalian apoB sequences were required for editing enhancement. In concert with the 29-nucleotide conserved cassette, the 5' rat apoB element (nucleotides 6615-6629) increased editing at C-6666, and was necessary for editing enhancement of chicken enterocyte S100 extracts. Similarly, the 3' rat apoB element (nucleotides 6726-6752) was required for editing enhancement of chicken enterocyte S100 extracts, but to a lesser extent in efficiency, compared to the 5' region. In conclusion, this study identified the sequences required for editing enhancement activity from chicken enterocyte S100 extracts.

Original languageEnglish
Pages (from-to)744-750
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume254
Issue number3
DOIs
StatePublished - Jan 27 1999
Externally publishedYes

Fingerprint Dive into the research topics of 'Sequence elements required for apolipoprotein B mRNA editing enhancement activity from chicken enterocytes'. Together they form a unique fingerprint.

Cite this