TY - JOUR
T1 - Sequence elements required for apolipoprotein B mRNA editing enhancement activity from chicken enterocytes
AU - Nakamuta, Makoto
AU - Tsai, An
AU - Chan, Lawrence
AU - Davidson, Nicholas O.
AU - Teng, Ba Bie
N1 - Funding Information:
This research was supported by National Institute of Health Grants HL-53441 (to BBT) and HL-56668 (to LC) and American Heart Association Grant-In-Aid (to BBT). We are grateful to Dr. Kunihisa Kobayashi from Baylor College of Medicine for the preparation of computer graphics. We are also grateful to Dr. Eva Zsig-mond from Institute of Molecular Medicine, University of Texas-Houston for critical reading this manuscript.
PY - 1999/1/27
Y1 - 1999/1/27
N2 - Mammalian intestinal apolipoprotein B (apoB) mRNA edits codon 2153 from CAA in apoB100 mRNA to a stop codon (UAA) in apoB48 mRNA. By contrast, chicken intestinal apoB mRNA contains a CAA codon at the corresponding site, but is not edited. Chicken enterocyte S100 extracts fail to edit mammalian apoB RNA, but contain factor(s) which enhance the mammalian enterocytes editing activity. By converting the chicken apoB mooring sequences to the conserved mammalian sequences, the study confirmed that this 11-nucleotide stretch was necessary and sufficient for minimal RNA editing. Using rat and chicken apoB chimeric constructs, the study revealed that mammalian apoB sequences were required for editing enhancement. In concert with the 29-nucleotide conserved cassette, the 5' rat apoB element (nucleotides 6615-6629) increased editing at C-6666, and was necessary for editing enhancement of chicken enterocyte S100 extracts. Similarly, the 3' rat apoB element (nucleotides 6726-6752) was required for editing enhancement of chicken enterocyte S100 extracts, but to a lesser extent in efficiency, compared to the 5' region. In conclusion, this study identified the sequences required for editing enhancement activity from chicken enterocyte S100 extracts.
AB - Mammalian intestinal apolipoprotein B (apoB) mRNA edits codon 2153 from CAA in apoB100 mRNA to a stop codon (UAA) in apoB48 mRNA. By contrast, chicken intestinal apoB mRNA contains a CAA codon at the corresponding site, but is not edited. Chicken enterocyte S100 extracts fail to edit mammalian apoB RNA, but contain factor(s) which enhance the mammalian enterocytes editing activity. By converting the chicken apoB mooring sequences to the conserved mammalian sequences, the study confirmed that this 11-nucleotide stretch was necessary and sufficient for minimal RNA editing. Using rat and chicken apoB chimeric constructs, the study revealed that mammalian apoB sequences were required for editing enhancement. In concert with the 29-nucleotide conserved cassette, the 5' rat apoB element (nucleotides 6615-6629) increased editing at C-6666, and was necessary for editing enhancement of chicken enterocyte S100 extracts. Similarly, the 3' rat apoB element (nucleotides 6726-6752) was required for editing enhancement of chicken enterocyte S100 extracts, but to a lesser extent in efficiency, compared to the 5' region. In conclusion, this study identified the sequences required for editing enhancement activity from chicken enterocyte S100 extracts.
UR - http://www.scopus.com/inward/record.url?scp=0033608218&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1998.9963
DO - 10.1006/bbrc.1998.9963
M3 - Article
C2 - 9920812
AN - SCOPUS:0033608218
SN - 0006-291X
VL - 254
SP - 744
EP - 750
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -