Sequence and S1 nuclease mapping of the 5′ region of the dihydrofolate reductasethymidylate synthase gene of Leishmania major

Geoffrey M. Kapler, Kang Zhang, Stephen M. Beverley

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The 5′ structure of mRNA transcribed from the dihydrofolate reductase-thymidylate synthase (DHFR-TS) gene of the protozoan parasite Leishmania major has been characterized. S1 nuclease mapping identifies a heterogenous 5′ structure which is not affected by growth phase or developmental stage. The DNA sequence of the 5′ region of the DHFR-TS gene does not reveal homology with other trypanosomatid genes, eukaryotic consensus genetic elements, or the mammalian DHFR promoter element. This latter finding is especially significant as we show that the 5′ region of the E. coli DHFR gene exhibits homology to the mammalian DHFR promoter element, despite their greater evolutionary distance.

Original languageEnglish
Pages (from-to)3369-3383
Number of pages15
JournalNucleic acids research
Volume15
Issue number8
DOIs
StatePublished - Apr 24 1987
Externally publishedYes

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