TY - JOUR
T1 - Sequence and S1 nuclease mapping of the 5′ region of the dihydrofolate reductasethymidylate synthase gene of Leishmania major
AU - Kapler, Geoffrey M.
AU - Zhang, Kang
AU - Beverley, Stephen M.
N1 - Funding Information:
ACKN0WLED6EHENTS He thank Jacqueline Miller forextensive discussionsand providing unpublished sequence data ofthebeta-tubulin andm1n1-exon o f l ^ <na_i.2.L' T o m Ellenberger f o r assistance 1 n D N A sequencing, S.Sazer, andD.Dobson. This work wassupportedby NIH Grant AI21903-02. GMKwassupported byNIHTraining Grant 5-T-32-GH07196 andKZbyHarvard University, Division of Medical Sciences. SM isa Burroughs-We11come Scholar 1n Molecular Parasi tology.
PY - 1987/4/24
Y1 - 1987/4/24
N2 - The 5′ structure of mRNA transcribed from the dihydrofolate reductase-thymidylate synthase (DHFR-TS) gene of the protozoan parasite Leishmania major has been characterized. S1 nuclease mapping identifies a heterogenous 5′ structure which is not affected by growth phase or developmental stage. The DNA sequence of the 5′ region of the DHFR-TS gene does not reveal homology with other trypanosomatid genes, eukaryotic consensus genetic elements, or the mammalian DHFR promoter element. This latter finding is especially significant as we show that the 5′ region of the E. coli DHFR gene exhibits homology to the mammalian DHFR promoter element, despite their greater evolutionary distance.
AB - The 5′ structure of mRNA transcribed from the dihydrofolate reductase-thymidylate synthase (DHFR-TS) gene of the protozoan parasite Leishmania major has been characterized. S1 nuclease mapping identifies a heterogenous 5′ structure which is not affected by growth phase or developmental stage. The DNA sequence of the 5′ region of the DHFR-TS gene does not reveal homology with other trypanosomatid genes, eukaryotic consensus genetic elements, or the mammalian DHFR promoter element. This latter finding is especially significant as we show that the 5′ region of the E. coli DHFR gene exhibits homology to the mammalian DHFR promoter element, despite their greater evolutionary distance.
UR - http://www.scopus.com/inward/record.url?scp=0023663448&partnerID=8YFLogxK
U2 - 10.1093/nar/15.8.3369
DO - 10.1093/nar/15.8.3369
M3 - Article
C2 - 3554143
AN - SCOPUS:0023663448
SN - 0305-1048
VL - 15
SP - 3369
EP - 3383
JO - Nucleic acids research
JF - Nucleic acids research
IS - 8
ER -