Sequence analysis and functional studies of interleukin-3 receptor α subunit-encoding cDNAs amplified from KG-1 leukemic cells and normal human marrow

Aaron P. Rapoport, John F. DiPersio

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Two partial cDNAs encoding the human interleukin-3 receptor a subunit (IL-3Rα) were cloned from KG-1 leukemic cells using the polymerase chain reaction. Sequence analysis of these cDNAs predicted two single-amino-acid (aa) changes as compared with the published TF-1 leukemic cell IL-3Rα sequence [Kitamura et al., Cell 66 (1991) 1165-1174]. These changes were confirmed by sequence analysis of a second set of independently derived cDNAs. Identical aa changes were found in the IL-3Rα encoded by cDNAs cloned from normal CD34+ human marrow cells. Ligation of the partial cDNAs derived from KG-1 cells resulted in a full-length functional IL-3Rα cDNA clone. Deletion of the extracellular 'LSXWS' consensus sequence resulted in complete loss of detectable [125I]IL-3 binding when this mutant receptor was co-expressed in COS-7 cells with the β subunit of the IL-3 receptor.

Original languageEnglish
Pages (from-to)333-337
Number of pages5
JournalGene
Volume137
Issue number2
DOIs
StatePublished - Dec 31 1993
Externally publishedYes

Keywords

  • Hematopoietic growth factors
  • amino acid polymorphism
  • cDNA cloning
  • cytokine receptor
  • mutational analysis
  • polymerase chain reaction

Fingerprint Dive into the research topics of 'Sequence analysis and functional studies of interleukin-3 receptor α subunit-encoding cDNAs amplified from KG-1 leukemic cells and normal human marrow'. Together they form a unique fingerprint.

  • Cite this