Separation of oligosaccharides using high-performance anion-exchange chromatography with pulsed amperometric detection

R. Reid townsend, Mark R. Hardy, Yuan Chuan Lee

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

This chapter focuses on the separation of oligosaccharides using high-performance anion-exchange (HPAE) chromatography with pulsed amperometric detection. The chapter discusses the results from coupling HPAE chromatography with pulsed amperometric detection (HPAE-PAD) for the chromatography and electrochemical quantification of underivatized oligosaccharides. Within a group of neutral oligosaccharides of approximately the same size and with larger sialylated oligosaccharides (degree of polymerization 14 to 16), the electrochemical response is a reasonably accurate measurement of oligosaccharide concentration. The response factors for phosphorylated oligosaccharides followed the same rules as that of neutral oligosaccharides with an additional decrease in response related to the number of phosphate residues. Within a group of neutral oligosaccharides of approximately the same size and with larger sialylated oligosaccharides (degree of polymerization 14 to 16), the electrochemical response is a reasonably accurate measurement of oligosaccharide concentration.

Original languageEnglish
Pages (from-to)65-76
Number of pages12
JournalMethods in enzymology
Volume179
Issue numberC
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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