TY - JOUR
T1 - Separation of neutral oligosaccharides by high-performance liquid chromatography
AU - Mellis, Scott J.
AU - Baenziger, Jacques U.
N1 - Funding Information:
’ This investigation was supported by Grant RO I CA 21923 awarded by the National Cancer Institute, DHEW, and Grant PCM 7911486 awarded by the National Science Foundation. J.U.B. is a recipient of a Research Career Development Award. Grant I K04 CA00671, from the National Cancer Institute, DHEW. is a Medical Scientist Trainee supported GM07200 from the National Institute of General Sciences. This study was also supported by the companies: Brown & Williamson Tobacco Philip Morris Incorporated; R.J. Reynolds Company: and United States Tobacco Com-
PY - 1981/7/1
Y1 - 1981/7/1
N2 - We have developed a method for separating reduced, neutral oligosaccharides by high-performance liquid chromatography on columns of MicroPak AX-5 (Varian Associates) with a mobile phase consisting of acetonitrile:H2O. Individual glucose oligomers containing from 1 to 20 glucose moieties can be separated in a single 1-h analysis with a solvent program of decreasing acetonitrile concentration. We have applied this method to both the analysis and preparative isolation of glycoprotein-derived oligosaccharides obtained by enzymatic release with endoglycosidases or chemical release by hydrazinolysis. Introduction of 3H by reduction with NaB3H4 permits the detection of subnanomole quantities of oligosaccharides. This method offers previously unattainable rapidity and resolution for the analysis of oligosaccharides.
AB - We have developed a method for separating reduced, neutral oligosaccharides by high-performance liquid chromatography on columns of MicroPak AX-5 (Varian Associates) with a mobile phase consisting of acetonitrile:H2O. Individual glucose oligomers containing from 1 to 20 glucose moieties can be separated in a single 1-h analysis with a solvent program of decreasing acetonitrile concentration. We have applied this method to both the analysis and preparative isolation of glycoprotein-derived oligosaccharides obtained by enzymatic release with endoglycosidases or chemical release by hydrazinolysis. Introduction of 3H by reduction with NaB3H4 permits the detection of subnanomole quantities of oligosaccharides. This method offers previously unattainable rapidity and resolution for the analysis of oligosaccharides.
UR - http://www.scopus.com/inward/record.url?scp=0019845279&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(81)90480-2
DO - 10.1016/0003-2697(81)90480-2
M3 - Article
C2 - 7304916
AN - SCOPUS:0019845279
VL - 114
SP - 276
EP - 280
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -