Separation of neutral oligosaccharides by high-performance liquid chromatography

Scott J. Mellis, Jacques U. Baenziger

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

We have developed a method for separating reduced, neutral oligosaccharides by high-performance liquid chromatography on columns of MicroPak AX-5 (Varian Associates) with a mobile phase consisting of acetonitrile:H2O. Individual glucose oligomers containing from 1 to 20 glucose moieties can be separated in a single 1-h analysis with a solvent program of decreasing acetonitrile concentration. We have applied this method to both the analysis and preparative isolation of glycoprotein-derived oligosaccharides obtained by enzymatic release with endoglycosidases or chemical release by hydrazinolysis. Introduction of 3H by reduction with NaB3H4 permits the detection of subnanomole quantities of oligosaccharides. This method offers previously unattainable rapidity and resolution for the analysis of oligosaccharides.

Original languageEnglish
Pages (from-to)276-280
Number of pages5
JournalAnalytical Biochemistry
Volume114
Issue number2
DOIs
StatePublished - Jul 1 1981

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