Separation of neutral oligosaccharides by high-performance liquid chromatography

Scott J. Mellis; Jacques U. Baenziger

doi:10.1016/0003-2697(81)90480-2

Separation of neutral oligosaccharides by high-performance liquid chromatography

Scott J. Mellis, Jacques U. Baenziger

Biochemistry and Molecular Biophysics

Research output: Contribution to journal › Article

92 Scopus citations

Abstract

We have developed a method for separating reduced, neutral oligosaccharides by high-performance liquid chromatography on columns of MicroPak AX-5 (Varian Associates) with a mobile phase consisting of acetonitrile:H₂O. Individual glucose oligomers containing from 1 to 20 glucose moieties can be separated in a single 1-h analysis with a solvent program of decreasing acetonitrile concentration. We have applied this method to both the analysis and preparative isolation of glycoprotein-derived oligosaccharides obtained by enzymatic release with endoglycosidases or chemical release by hydrazinolysis. Introduction of ³H by reduction with NaB³H₄ permits the detection of subnanomole quantities of oligosaccharides. This method offers previously unattainable rapidity and resolution for the analysis of oligosaccharides.

Original language	English
Pages (from-to)	276-280
Number of pages	5
Journal	Analytical Biochemistry
Volume	114
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(81)90480-2
State	Published - Jul 1 1981

Access to Document

10.1016/0003-2697(81)90480-2

Link to publication in Scopus

Fingerprint Dive into the research topics of 'Separation of neutral oligosaccharides by high-performance liquid chromatography'. Together they form a unique fingerprint.

Cite this

Mellis, S. J., & Baenziger, J. U. (1981). Separation of neutral oligosaccharides by high-performance liquid chromatography. Analytical Biochemistry, 114(2), 276-280. https://doi.org/10.1016/0003-2697(81)90480-2

@article{8d0698bf45724716939c070b0509a92b,

title = "Separation of neutral oligosaccharides by high-performance liquid chromatography",

abstract = "We have developed a method for separating reduced, neutral oligosaccharides by high-performance liquid chromatography on columns of MicroPak AX-5 (Varian Associates) with a mobile phase consisting of acetonitrile:H2O. Individual glucose oligomers containing from 1 to 20 glucose moieties can be separated in a single 1-h analysis with a solvent program of decreasing acetonitrile concentration. We have applied this method to both the analysis and preparative isolation of glycoprotein-derived oligosaccharides obtained by enzymatic release with endoglycosidases or chemical release by hydrazinolysis. Introduction of 3H by reduction with NaB3H4 permits the detection of subnanomole quantities of oligosaccharides. This method offers previously unattainable rapidity and resolution for the analysis of oligosaccharides.",

author = "Mellis, {Scott J.} and Baenziger, {Jacques U.}",

year = "1981",

month = jul,

day = "1",

doi = "10.1016/0003-2697(81)90480-2",

language = "English",

volume = "114",

pages = "276--280",

journal = "Analytical Biochemistry",

issn = "0003-2697",

number = "2",

}

TY - JOUR

T1 - Separation of neutral oligosaccharides by high-performance liquid chromatography

AU - Mellis, Scott J.

AU - Baenziger, Jacques U.

PY - 1981/7/1

Y1 - 1981/7/1

N2 - We have developed a method for separating reduced, neutral oligosaccharides by high-performance liquid chromatography on columns of MicroPak AX-5 (Varian Associates) with a mobile phase consisting of acetonitrile:H2O. Individual glucose oligomers containing from 1 to 20 glucose moieties can be separated in a single 1-h analysis with a solvent program of decreasing acetonitrile concentration. We have applied this method to both the analysis and preparative isolation of glycoprotein-derived oligosaccharides obtained by enzymatic release with endoglycosidases or chemical release by hydrazinolysis. Introduction of 3H by reduction with NaB3H4 permits the detection of subnanomole quantities of oligosaccharides. This method offers previously unattainable rapidity and resolution for the analysis of oligosaccharides.

AB - We have developed a method for separating reduced, neutral oligosaccharides by high-performance liquid chromatography on columns of MicroPak AX-5 (Varian Associates) with a mobile phase consisting of acetonitrile:H2O. Individual glucose oligomers containing from 1 to 20 glucose moieties can be separated in a single 1-h analysis with a solvent program of decreasing acetonitrile concentration. We have applied this method to both the analysis and preparative isolation of glycoprotein-derived oligosaccharides obtained by enzymatic release with endoglycosidases or chemical release by hydrazinolysis. Introduction of 3H by reduction with NaB3H4 permits the detection of subnanomole quantities of oligosaccharides. This method offers previously unattainable rapidity and resolution for the analysis of oligosaccharides.

UR - http://www.scopus.com/inward/record.url?scp=0019845279&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(81)90480-2

DO - 10.1016/0003-2697(81)90480-2

M3 - Article

C2 - 7304916

AN - SCOPUS:0019845279

VL - 114

SP - 276

EP - 280

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -