Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique

Sherif Helmy; Kathleen Porter-Jordan; E. A. Dawidowicz; Paul Pilch; Alan L. Schwartz; Richard E. Fine

doi:10.1016/0092-8674(86)90471-X

Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique

Sherif Helmy, Kathleen Porter-Jordan, E. A. Dawidowicz, Paul Pilch, Alan L. Schwartz, Richard E. Fine

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Coated vesicles isolated from rat liver perfused with diisopropylfluorophosphate (DFP) to inactivate endogenous cholinesterase contained newly synthesized secretory cholinesterase after a 30 min recovery. The cholinesterase is found in coated vesicles of presumed endocytic origin following DFP treatment and perfusion for 3 min with galactosylated cholinesterase, a ligand for the asialoglycoprotein recpetor. Highly enriched populations of endocytic and exocytic coated vesicles can be separated by use of a novel cholinesterase mediated density shift technique. The two coated vesicle classes have very similar polypeptide compositions but differ significantly in the ratio of cholesterol to phospholipid.

Original language	English
Pages (from-to)	497-506
Number of pages	10
Journal	Cell
Volume	44
Issue number	3
DOIs	https://doi.org/10.1016/0092-8674(86)90471-X
State	Published - Feb 14 1986

Access to Document

10.1016/0092-8674(86)90471-X

Cite this

@article{b7cae7cd73f24cd186f2e67fa561665f,

title = "Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique",

abstract = "Coated vesicles isolated from rat liver perfused with diisopropylfluorophosphate (DFP) to inactivate endogenous cholinesterase contained newly synthesized secretory cholinesterase after a 30 min recovery. The cholinesterase is found in coated vesicles of presumed endocytic origin following DFP treatment and perfusion for 3 min with galactosylated cholinesterase, a ligand for the asialoglycoprotein recpetor. Highly enriched populations of endocytic and exocytic coated vesicles can be separated by use of a novel cholinesterase mediated density shift technique. The two coated vesicle classes have very similar polypeptide compositions but differ significantly in the ratio of cholesterol to phospholipid.",

author = "Sherif Helmy and Kathleen Porter-Jordan and Dawidowicz, {E. A.} and Paul Pilch and Schwartz, {Alan L.} and Fine, {Richard E.}",

note = "Funding Information: We would like to thank Michaelle Montgomery for expert secretarial sistance. This research was supported by grants ROl NIA10562 ROl AM33628 from the National Institutes of Health.",

year = "1986",

month = feb,

day = "14",

doi = "10.1016/0092-8674(86)90471-X",

language = "English",

volume = "44",

pages = "497--506",

journal = "Cell",

issn = "0092-8674",

number = "3",

}

TY - JOUR

T1 - Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique

AU - Helmy, Sherif

AU - Porter-Jordan, Kathleen

AU - Dawidowicz, E. A.

AU - Pilch, Paul

AU - Schwartz, Alan L.

AU - Fine, Richard E.

N1 - Funding Information: We would like to thank Michaelle Montgomery for expert secretarial sistance. This research was supported by grants ROl NIA10562 ROl AM33628 from the National Institutes of Health.

PY - 1986/2/14

Y1 - 1986/2/14

N2 - Coated vesicles isolated from rat liver perfused with diisopropylfluorophosphate (DFP) to inactivate endogenous cholinesterase contained newly synthesized secretory cholinesterase after a 30 min recovery. The cholinesterase is found in coated vesicles of presumed endocytic origin following DFP treatment and perfusion for 3 min with galactosylated cholinesterase, a ligand for the asialoglycoprotein recpetor. Highly enriched populations of endocytic and exocytic coated vesicles can be separated by use of a novel cholinesterase mediated density shift technique. The two coated vesicle classes have very similar polypeptide compositions but differ significantly in the ratio of cholesterol to phospholipid.

AB - Coated vesicles isolated from rat liver perfused with diisopropylfluorophosphate (DFP) to inactivate endogenous cholinesterase contained newly synthesized secretory cholinesterase after a 30 min recovery. The cholinesterase is found in coated vesicles of presumed endocytic origin following DFP treatment and perfusion for 3 min with galactosylated cholinesterase, a ligand for the asialoglycoprotein recpetor. Highly enriched populations of endocytic and exocytic coated vesicles can be separated by use of a novel cholinesterase mediated density shift technique. The two coated vesicle classes have very similar polypeptide compositions but differ significantly in the ratio of cholesterol to phospholipid.

UR - http://www.scopus.com/inward/record.url?scp=0022531437&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(86)90471-X

DO - 10.1016/0092-8674(86)90471-X

M3 - Article

C2 - 2867830

AN - SCOPUS:0022531437

SN - 0092-8674

VL - 44

SP - 497

EP - 506

JO - Cell

JF - Cell

IS - 3

ER -

Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique

Abstract

Access to Document

Other files and links

Fingerprint

Cite this