Separation of endocytic from exocytic coated vesicles using a novel cholinesterase mediated density shift technique

Sherif Helmy, Kathleen Porter-Jordan, E. A. Dawidowicz, Paul Pilch, Alan L. Schwartz, Richard E. Fine

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Coated vesicles isolated from rat liver perfused with diisopropylfluorophosphate (DFP) to inactivate endogenous cholinesterase contained newly synthesized secretory cholinesterase after a 30 min recovery. The cholinesterase is found in coated vesicles of presumed endocytic origin following DFP treatment and perfusion for 3 min with galactosylated cholinesterase, a ligand for the asialoglycoprotein recpetor. Highly enriched populations of endocytic and exocytic coated vesicles can be separated by use of a novel cholinesterase mediated density shift technique. The two coated vesicle classes have very similar polypeptide compositions but differ significantly in the ratio of cholesterol to phospholipid.

Original languageEnglish
Pages (from-to)497-506
Number of pages10
JournalCell
Volume44
Issue number3
DOIs
StatePublished - Feb 14 1986
Externally publishedYes

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