Sensitivity and kinetics of signal transmission at the first visual synapse differentially impact Visually-Guided behavior

Ignacio Sarria, Johan Pahlberg, Yan Cao, Alexander V. Kolesnikov, Vladimir J. Kefalov, Alapakkam P. Sampath, Kirill A. Martemyanov

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

In the retina, synaptic transmission between photoreceptors and downstream ON-bipolar neurons (ON-BCs) is mediated by a GPCR pathway, which plays an essential role in vision. However, the mechanisms that control signal transmission at this synapse and its relevance to behavior remain poorly understood. In this study we used a genetic system to titrate the rate of GPCR signaling in ON- BC dendrites by varying the concentration of key RGS proteins and measuring the impact on transmission of signal between photoreceptors and ON-BC neurons using electroretinography and single cell recordings. We found that sensitivity, onset timing, and the maximal amplitude of light- evoked responses in rod- and cone-driven ON-BCs are determined by different RGS concentrations. We further show that changes in RGS concentration differentially impact visually guided-behavior mediated by rod and cone ON pathways. These findings illustrate that neuronal circuit properties can be modulated by adjusting parameters of GPCR-based neurotransmission at individual synapses.

Original languageEnglish
Article numbere06358
JournaleLife
Volume2015
Issue number4
DOIs
StatePublished - Apr 16 2015

Fingerprint Dive into the research topics of 'Sensitivity and kinetics of signal transmission at the first visual synapse differentially impact Visually-Guided behavior'. Together they form a unique fingerprint.

  • Cite this

    Sarria, I., Pahlberg, J., Cao, Y., Kolesnikov, A. V., Kefalov, V. J., Sampath, A. P., & Martemyanov, K. A. (2015). Sensitivity and kinetics of signal transmission at the first visual synapse differentially impact Visually-Guided behavior. eLife, 2015(4), [e06358]. https://doi.org/10.7554/eLife.06358