Self-renewing resident cardiac macrophages limit adverse remodeling following myocardial infarction

Sarah A. Dick, Jillian A. Macklin, Sara Nejat, Abdul Momen, Xavier Clemente-Casares, Marwan G. Althagafi, Jinmiao Chen, Crystal Kantores, Siyavash Hosseinzadeh, Laura Aronoff, Anthony Wong, Rysa Zaman, Iulia Barbu, Rickvinder Besla, Kory J. Lavine, Babak Razani, Florent Ginhoux, Mansoor Husain, Myron I. Cybulsky, Clinton S. RobbinsSlava Epelman

Research output: Contribution to journalArticlepeer-review

158 Scopus citations

Abstract

Macrophages promote both injury and repair after myocardial infarction, but discriminating functions within mixed populations remains challenging. Here we used fate mapping, parabiosis and single-cell transcriptomics to demonstrate that at steady state, TIMD4+LYVE1+MHC-IIloCCR2 resident cardiac macrophages self-renew with negligible blood monocyte input. Monocytes partially replaced resident TIMD4LYVE1MHC-IIhiCCR2 macrophages and fully replaced TIMD4LYVE1MHC-IIhiCCR2+ macrophages, revealing a hierarchy of monocyte contribution to functionally distinct macrophage subsets. Ischemic injury reduced TIMD4+ and TIMD4 resident macrophage abundance, whereas CCR2+ monocyte-derived macrophages adopted multiple cell fates within infarcted tissue, including those nearly indistinguishable from resident macrophages. Recruited macrophages did not express TIMD4, highlighting the ability of TIMD4 to track a subset of resident macrophages in the absence of fate mapping. Despite this similarity, inducible depletion of resident macrophages using a Cx3cr1-based system led to impaired cardiac function and promoted adverse remodeling primarily within the peri-infarct zone, revealing a nonredundant, cardioprotective role of resident cardiac macrophages.

Original languageEnglish
Pages (from-to)29-39
Number of pages11
JournalNature immunology
Volume20
Issue number1
DOIs
StatePublished - Jan 1 2019

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