TY - JOUR
T1 - Selective phosphorylation of the IgE receptor in antigen-stimulated rat mast cells
AU - Hempstead, B. L.
AU - Parker, C. W.
AU - Kulczycki, A.
PY - 1983
Y1 - 1983
N2 - Purified rat serosal mast cells were sensitized with mouse immunoglobulin E (IgE) anti-2,4-dinitrophenyl antibody, partially depleted of phosphate, labeled with [32P]orthophosphate, and stimulated with dinitrophenylated bovine serum albumin or control protein. After 15-120 seconds at 37°C, the cells were extracted with nonionic detergent. IgE receptors were purified by repetitive affinity chromatography and were analyzed by NaDodSO4/polyacrylamide gel electrophoresis and radioautography. Antigenic stimulation of intact rat mast cells produced a rapid and marked increase in the phosphorylation of the surface-exposed α component of the IgE receptor. However, phosphorylation of the 33,000 M(r) β component of the IgE receptor was not altered significantly by antigen stimulation. This suggests that the selective increase in phosphorylation of the IgE receptor α component may be part of the physiologic mediator secretion process triggered by antigen.
AB - Purified rat serosal mast cells were sensitized with mouse immunoglobulin E (IgE) anti-2,4-dinitrophenyl antibody, partially depleted of phosphate, labeled with [32P]orthophosphate, and stimulated with dinitrophenylated bovine serum albumin or control protein. After 15-120 seconds at 37°C, the cells were extracted with nonionic detergent. IgE receptors were purified by repetitive affinity chromatography and were analyzed by NaDodSO4/polyacrylamide gel electrophoresis and radioautography. Antigenic stimulation of intact rat mast cells produced a rapid and marked increase in the phosphorylation of the surface-exposed α component of the IgE receptor. However, phosphorylation of the 33,000 M(r) β component of the IgE receptor was not altered significantly by antigen stimulation. This suggests that the selective increase in phosphorylation of the IgE receptor α component may be part of the physiologic mediator secretion process triggered by antigen.
UR - http://www.scopus.com/inward/record.url?scp=0020639123&partnerID=8YFLogxK
U2 - 10.1073/pnas.80.10.3050
DO - 10.1073/pnas.80.10.3050
M3 - Article
C2 - 6190168
AN - SCOPUS:0020639123
SN - 0027-8424
VL - 80
SP - 3050
EP - 3053
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 10 I
ER -