Selective phosphorylation of the IgE receptor in antigen-stimulated rat mast cells

B. L. Hempstead, C. W. Parker, A. Kulczycki

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Purified rat serosal mast cells were sensitized with mouse immunoglobulin E (IgE) anti-2,4-dinitrophenyl antibody, partially depleted of phosphate, labeled with [32P]orthophosphate, and stimulated with dinitrophenylated bovine serum albumin or control protein. After 15-120 seconds at 37°C, the cells were extracted with nonionic detergent. IgE receptors were purified by repetitive affinity chromatography and were analyzed by NaDodSO4/polyacrylamide gel electrophoresis and radioautography. Antigenic stimulation of intact rat mast cells produced a rapid and marked increase in the phosphorylation of the surface-exposed α component of the IgE receptor. However, phosphorylation of the 33,000 M(r) β component of the IgE receptor was not altered significantly by antigen stimulation. This suggests that the selective increase in phosphorylation of the IgE receptor α component may be part of the physiologic mediator secretion process triggered by antigen.

Original languageEnglish
Pages (from-to)3050-3053
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number10 I
DOIs
StatePublished - Jan 1 1983

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