Selective inhibition of photosystem II in spinach by tobacco mosaic virus: An effect of the viral coat protein

Richard A.J. Hodgson; Roger N. Beachy; Himadri B. Pakrasi

doi:10.1016/0014-5793(89)80234-0

Selective inhibition of photosystem II in spinach by tobacco mosaic virus: An effect of the viral coat protein

Richard A.J. Hodgson, Roger N. Beachy, Himadri B. Pakrasi

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

Leaves of Spinacia oleracea inoculated with tobacco mosaic virus (TMV) strain PV230 develop mild chlorotic and mosaic symptoms of infection. Thylakoid membranes isolated from these infected leaves showed a reduced F_v/F_m ratio for chlorophyll fluorescence kinetics, at 25°C. The photosystem II (PS Il)-mediated electron-transport rate was inhibited 50%, whereas PS I activity was unaffected by virus infection. Protein analysis indicated that TMV coat protein was associated with thylakoids, in particular with the PS II fraction. The results demonstrate that TMV-infected S. oleracea shows inhibition of photosynthetic electron transport through PS II. We propose that the inhibition of photosynthetic activity results from the association of viral coat protein with the PS II complex.

Original language	English
Pages (from-to)	267-270
Number of pages	4
Journal	FEBS Letters
Volume	245
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(89)80234-0
State	Published - Mar 13 1989

Keywords

(Spinacia oleracea)
Photosynthesis inhibition
Photosystem II
TMV
Viral coat protein

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10.1016/0014-5793(89)80234-0

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@article{33b0c83ff4d54c35b257863e05a0604e,

title = "Selective inhibition of photosystem II in spinach by tobacco mosaic virus: An effect of the viral coat protein",

abstract = "Leaves of Spinacia oleracea inoculated with tobacco mosaic virus (TMV) strain PV230 develop mild chlorotic and mosaic symptoms of infection. Thylakoid membranes isolated from these infected leaves showed a reduced Fv/Fm ratio for chlorophyll fluorescence kinetics, at 25°C. The photosystem II (PS Il)-mediated electron-transport rate was inhibited 50%, whereas PS I activity was unaffected by virus infection. Protein analysis indicated that TMV coat protein was associated with thylakoids, in particular with the PS II fraction. The results demonstrate that TMV-infected S. oleracea shows inhibition of photosynthetic electron transport through PS II. We propose that the inhibition of photosynthetic activity results from the association of viral coat protein with the PS II complex.",

keywords = "(Spinacia oleracea), Photosynthesis inhibition, Photosystem II, TMV, Viral coat protein",

author = "Hodgson, {Richard A.J.} and Beachy, {Roger N.} and Pakrasi, {Himadri B.}",

note = "Funding Information: AcknowledgementWs:e would like to thankD r DeweyH olten for the useo f a kineticf luorometerR. .A.J.H. wass upportedb y the Monsanto Research Fellowship Funds at Washington University. Additional funding was provided by BRSG SO7 RR07054-23,a warded to H.B.P. by the Institutional Biomedical ResearchS upport Grant Program, Division of Re-searchR esources,N ational Instituteso f Health and by a re-searchg rantf rom MonsantoC o. to R.N.B.",

year = "1989",

month = mar,

day = "13",

doi = "10.1016/0014-5793(89)80234-0",

language = "English",

volume = "245",

pages = "267--270",

journal = "FEBS Letters",

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number = "1-2",

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TY - JOUR

T1 - Selective inhibition of photosystem II in spinach by tobacco mosaic virus

T2 - An effect of the viral coat protein

AU - Hodgson, Richard A.J.

AU - Beachy, Roger N.

AU - Pakrasi, Himadri B.

N1 - Funding Information: AcknowledgementWs:e would like to thankD r DeweyH olten for the useo f a kineticf luorometerR. .A.J.H. wass upportedb y the Monsanto Research Fellowship Funds at Washington University. Additional funding was provided by BRSG SO7 RR07054-23,a warded to H.B.P. by the Institutional Biomedical ResearchS upport Grant Program, Division of Re-searchR esources,N ational Instituteso f Health and by a re-searchg rantf rom MonsantoC o. to R.N.B.

PY - 1989/3/13

Y1 - 1989/3/13

N2 - Leaves of Spinacia oleracea inoculated with tobacco mosaic virus (TMV) strain PV230 develop mild chlorotic and mosaic symptoms of infection. Thylakoid membranes isolated from these infected leaves showed a reduced Fv/Fm ratio for chlorophyll fluorescence kinetics, at 25°C. The photosystem II (PS Il)-mediated electron-transport rate was inhibited 50%, whereas PS I activity was unaffected by virus infection. Protein analysis indicated that TMV coat protein was associated with thylakoids, in particular with the PS II fraction. The results demonstrate that TMV-infected S. oleracea shows inhibition of photosynthetic electron transport through PS II. We propose that the inhibition of photosynthetic activity results from the association of viral coat protein with the PS II complex.

AB - Leaves of Spinacia oleracea inoculated with tobacco mosaic virus (TMV) strain PV230 develop mild chlorotic and mosaic symptoms of infection. Thylakoid membranes isolated from these infected leaves showed a reduced Fv/Fm ratio for chlorophyll fluorescence kinetics, at 25°C. The photosystem II (PS Il)-mediated electron-transport rate was inhibited 50%, whereas PS I activity was unaffected by virus infection. Protein analysis indicated that TMV coat protein was associated with thylakoids, in particular with the PS II fraction. The results demonstrate that TMV-infected S. oleracea shows inhibition of photosynthetic electron transport through PS II. We propose that the inhibition of photosynthetic activity results from the association of viral coat protein with the PS II complex.

KW - (Spinacia oleracea)

KW - Photosynthesis inhibition

KW - Photosystem II

KW - TMV

KW - Viral coat protein

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U2 - 10.1016/0014-5793(89)80234-0

DO - 10.1016/0014-5793(89)80234-0

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C2 - 2924924

AN - SCOPUS:0024968245

SN - 0014-5793

VL - 245

SP - 267

EP - 270

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Selective inhibition of photosystem II in spinach by tobacco mosaic virus: An effect of the viral coat protein

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