Selective inhibition of photosystem II in spinach by tobacco mosaic virus: An effect of the viral coat protein

Richard A.J. Hodgson, Roger N. Beachy, Himadri B. Pakrasi

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

Leaves of Spinacia oleracea inoculated with tobacco mosaic virus (TMV) strain PV230 develop mild chlorotic and mosaic symptoms of infection. Thylakoid membranes isolated from these infected leaves showed a reduced Fv/Fm ratio for chlorophyll fluorescence kinetics, at 25°C. The photosystem II (PS Il)-mediated electron-transport rate was inhibited 50%, whereas PS I activity was unaffected by virus infection. Protein analysis indicated that TMV coat protein was associated with thylakoids, in particular with the PS II fraction. The results demonstrate that TMV-infected S. oleracea shows inhibition of photosynthetic electron transport through PS II. We propose that the inhibition of photosynthetic activity results from the association of viral coat protein with the PS II complex.

Original languageEnglish
Pages (from-to)267-270
Number of pages4
JournalFEBS Letters
Volume245
Issue number1-2
DOIs
StatePublished - Mar 13 1989

Keywords

  • (Spinacia oleracea)
  • Photosynthesis inhibition
  • Photosystem II
  • TMV
  • Viral coat protein

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