The incubation of suspensions of canine tracheal epithelial cells of greater than 95% purity with arachidonic acid (25-200 μg/ml) for 60-120 min resulted in the generation of a maximum of 36.2 ± 9.1 picomoles of leukotriene B4 106 cells, less than 2.0 picomoles of leukotrienes C4, D4, and E4 106 cells, and 1030 ± 463, 767 ± 500, and 324 ± 100 picomoles/106 cells of 15-, 12-, and 5-hydroxy-eicosatetraenoic acids, respectively (mean ± SEM, n = 8). The identity of leukotriene B4 was established by chromatographic and spectral properties, by reactivity with mono-specific anti-plasma, and by the chemotactic activity for neutrophils. Thus, the epithelium may be an important source of mediators of inflammation and hypersensitivity of pulmonary airways.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 12 1983|