Selective generation of leukotriene B4 by tracheal epithelial cells from dogs

M. J. Holtzman, H. Aizawa, J. A. Nadel, E. J. Goetzl

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

The incubation of suspensions of canine tracheal epithelial cells of greater than 95% purity with arachidonic acid (25-200 μg/ml) for 60-120 min resulted in the generation of a maximum of 36.2 ± 9.1 picomoles of leukotriene B4 106 cells, less than 2.0 picomoles of leukotrienes C4, D4, and E4 106 cells, and 1030 ± 463, 767 ± 500, and 324 ± 100 picomoles/106 cells of 15-, 12-, and 5-hydroxy-eicosatetraenoic acids, respectively (mean ± SEM, n = 8). The identity of leukotriene B4 was established by chromatographic and spectral properties, by reactivity with mono-specific anti-plasma, and by the chemotactic activity for neutrophils. Thus, the epithelium may be an important source of mediators of inflammation and hypersensitivity of pulmonary airways.

Original languageEnglish
Pages (from-to)1071-1076
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume114
Issue number3
DOIs
StatePublished - Aug 12 1983
Externally publishedYes

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