Selective generation of leukotriene B4 by tracheal epithelial cells from dogs

M. J. Holtzman; H. Aizawa; J. A. Nadel; E. J. Goetzl

doi:10.1016/0006-291X(83)90671-X

Selective generation of leukotriene B₄ by tracheal epithelial cells from dogs

M. J. Holtzman, H. Aizawa, J. A. Nadel, E. J. Goetzl

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Abstract

The incubation of suspensions of canine tracheal epithelial cells of greater than 95% purity with arachidonic acid (25-200 μg/ml) for 60-120 min resulted in the generation of a maximum of 36.2 ± 9.1 picomoles of leukotriene B₄ 10⁶ cells, less than 2.0 picomoles of leukotrienes C₄, D₄, and E₄ 10⁶ cells, and 1030 ± 463, 767 ± 500, and 324 ± 100 picomoles/10⁶ cells of 15-, 12-, and 5-hydroxy-eicosatetraenoic acids, respectively (mean ± SEM, n = 8). The identity of leukotriene B₄ was established by chromatographic and spectral properties, by reactivity with mono-specific anti-plasma, and by the chemotactic activity for neutrophils. Thus, the epithelium may be an important source of mediators of inflammation and hypersensitivity of pulmonary airways.

Original language	English
Pages (from-to)	1071-1076
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	114
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(83)90671-X
State	Published - Aug 12 1983

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title = "Selective generation of leukotriene B4 by tracheal epithelial cells from dogs",

abstract = "The incubation of suspensions of canine tracheal epithelial cells of greater than 95% purity with arachidonic acid (25-200 μg/ml) for 60-120 min resulted in the generation of a maximum of 36.2 ± 9.1 picomoles of leukotriene B4 106 cells, less than 2.0 picomoles of leukotrienes C4, D4, and E4 106 cells, and 1030 ± 463, 767 ± 500, and 324 ± 100 picomoles/106 cells of 15-, 12-, and 5-hydroxy-eicosatetraenoic acids, respectively (mean ± SEM, n = 8). The identity of leukotriene B4 was established by chromatographic and spectral properties, by reactivity with mono-specific anti-plasma, and by the chemotactic activity for neutrophils. Thus, the epithelium may be an important source of mediators of inflammation and hypersensitivity of pulmonary airways.",

author = "Holtzman, {M. J.} and H. Aizawa and Nadel, {J. A.} and Goetzl, {E. J.}",

note = "Funding Information: This work was supported by NIH Grants HL-24136, HL-00797, and HL-AM 31809, and by grants from the Council for Tobacco Research and California Air Resources Board.",

year = "1983",

month = aug,

day = "12",

doi = "10.1016/0006-291X(83)90671-X",

language = "English",

volume = "114",

pages = "1071--1076",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Selective generation of leukotriene B4 by tracheal epithelial cells from dogs

AU - Holtzman, M. J.

AU - Aizawa, H.

AU - Nadel, J. A.

AU - Goetzl, E. J.

N1 - Funding Information: This work was supported by NIH Grants HL-24136, HL-00797, and HL-AM 31809, and by grants from the Council for Tobacco Research and California Air Resources Board.

PY - 1983/8/12

Y1 - 1983/8/12

N2 - The incubation of suspensions of canine tracheal epithelial cells of greater than 95% purity with arachidonic acid (25-200 μg/ml) for 60-120 min resulted in the generation of a maximum of 36.2 ± 9.1 picomoles of leukotriene B4 106 cells, less than 2.0 picomoles of leukotrienes C4, D4, and E4 106 cells, and 1030 ± 463, 767 ± 500, and 324 ± 100 picomoles/106 cells of 15-, 12-, and 5-hydroxy-eicosatetraenoic acids, respectively (mean ± SEM, n = 8). The identity of leukotriene B4 was established by chromatographic and spectral properties, by reactivity with mono-specific anti-plasma, and by the chemotactic activity for neutrophils. Thus, the epithelium may be an important source of mediators of inflammation and hypersensitivity of pulmonary airways.

AB - The incubation of suspensions of canine tracheal epithelial cells of greater than 95% purity with arachidonic acid (25-200 μg/ml) for 60-120 min resulted in the generation of a maximum of 36.2 ± 9.1 picomoles of leukotriene B4 106 cells, less than 2.0 picomoles of leukotrienes C4, D4, and E4 106 cells, and 1030 ± 463, 767 ± 500, and 324 ± 100 picomoles/106 cells of 15-, 12-, and 5-hydroxy-eicosatetraenoic acids, respectively (mean ± SEM, n = 8). The identity of leukotriene B4 was established by chromatographic and spectral properties, by reactivity with mono-specific anti-plasma, and by the chemotactic activity for neutrophils. Thus, the epithelium may be an important source of mediators of inflammation and hypersensitivity of pulmonary airways.

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U2 - 10.1016/0006-291X(83)90671-X

DO - 10.1016/0006-291X(83)90671-X

M3 - Article

C2 - 6311194

AN - SCOPUS:0021101694

SN - 0006-291X

VL - 114

SP - 1071

EP - 1076

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Selective generation of leukotriene B₄ by tracheal epithelial cells from dogs

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