TY - JOUR
T1 - Selective expression of a subset of measles virus receptor-competent CD46 isoforms in human brain
AU - Buchholz, Christian J.
AU - Gerlier, Denis
AU - Hu, Aizhong
AU - Cathomen, Toni
AU - Liszewski, M. Kathryn
AU - Atkinson, John P.
AU - Cattaneo, Roberto
N1 - Funding Information:
C.J.B. received an EMBO short-term fellowship during his stay in Lyon. The authors wish to thank M. A. Billeter for helpful discussions, K. Baczko, J. Lampe, A. Aguzzi, A. Fontana, and V. ter Meulen for brain autopsy material, and F. Ochsenbein for the photographs. This work was supported by Grants 31-29343.90 and 31-33746.92 from the Schweizerische Nationalfonds (R.C.), CNRS-ATIPE, and Fondation pour la Recherche Médicale (D.G.).
PY - 1996/3/1
Y1 - 1996/3/1
N2 - The human cell surface protein CD46 is the main measles virus (MV) receptor. We analyzed the CD46 isoforms expressed in the brain of three patients who died with persistent MV infections and in an unaffected brain. Complete CD46 cDNAs were produced and found to code exclusively for CD46 isoforms with cytoplasmic tail 2. Selective expression of tail 2 isoforms was shown in a second control brain by Western blots with antibodies specific for each of the cytoplasmic tails. Binding of purified MV particles and virus-dependent cell fusion were tested after transient expression of brain-derived CD46 proteins in mouse cells. All the brain-derived proteins mediated MV binding and virus-dependent fusion. Isoforms containing both serine/threonine/proline (STP)-rich domains were more active in virus binding, whereas isoforms with only one STP domain were more efficient in mediating fusion.
AB - The human cell surface protein CD46 is the main measles virus (MV) receptor. We analyzed the CD46 isoforms expressed in the brain of three patients who died with persistent MV infections and in an unaffected brain. Complete CD46 cDNAs were produced and found to code exclusively for CD46 isoforms with cytoplasmic tail 2. Selective expression of tail 2 isoforms was shown in a second control brain by Western blots with antibodies specific for each of the cytoplasmic tails. Binding of purified MV particles and virus-dependent cell fusion were tested after transient expression of brain-derived CD46 proteins in mouse cells. All the brain-derived proteins mediated MV binding and virus-dependent fusion. Isoforms containing both serine/threonine/proline (STP)-rich domains were more active in virus binding, whereas isoforms with only one STP domain were more efficient in mediating fusion.
UR - http://www.scopus.com/inward/record.url?scp=0030006144&partnerID=8YFLogxK
U2 - 10.1006/viro.1996.0122
DO - 10.1006/viro.1996.0122
M3 - Article
C2 - 8599221
AN - SCOPUS:0030006144
SN - 0042-6822
VL - 217
SP - 349
EP - 355
JO - Virology
JF - Virology
IS - 1
ER -