Seasonal nitrogen remobilization and the role of auxin transport in poplar trees

Gen Li, Rongshoung Lin, Chioma Egekwu, Joshua Blakeslee, Jinshan Lin, Emily Pettengill, Angus S. Murphy, Wendy A. Peer, Nazrul Islam, Benjamin A. Babst, Fei Gao, Sergey Komarov, Yuan Chuan Tai, Gary D. Coleman

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Seasonal nitrogen (N) cycling in Populus, involves bark storage proteins (BSPs) that accumulate in bark phloem parenchyma in the autumn and decline when shoot growth resumes in the spring. Little is known about the contribution of BSPs to growth or the signals regulating N remobilization from BSPs. Knockdown of BSP accumulation via RNAi and N sink manipulations were used to understand how BSP storage influences shoot growth. Reduced accumulation of BSPs delayed bud break and reduced shoot growth following dormancy. Further, 13N tracer studies also showed that BSP accumulation is an important factor in N partitioning from senescing leaves to bark. Thus, BSP accumulation has a role in N remobilization during N partitioning both from senescing leaves to bark and from bark to expanding shoots once growth commences following dormancy. The bark transcriptome during BSP catabolism and N remobilization was enriched in genes associated with auxin transport and signaling, and manipulation of the source of auxin or auxin transport revealed a role for auxin in regulating BSP catabolism and N remobilization. Therefore, N remobilization appears to be regulated by auxin produced in expanding buds and shoots that is transported to bark where it regulates protease gene expression and BSP catabolism.

Original languageEnglish
Pages (from-to)4512-4530
Number of pages19
JournalJournal of Experimental Botany
Volume71
Issue number15
DOIs
StatePublished - Jul 25 2020

Keywords

  • Auxin
  • Bark storage proteins
  • Nitrogen remobilization
  • Populus
  • Seasonal nitrogen cycling

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