@article{7408ab73558d4132a4c40a9e5fef0148,
title = "Scanning electron microscopic appearance of chronic ulcerative colitis with and without dysplasia",
abstract = "This study was conducted to determine whether scanning electron microscopy of colonic mucosal biopsy specimens can help to detect dysplasia in patients with chronic ulcerative colitis. In the first phase of the study, using light microscopy as the standard for the diagnosis, the scanning electron microscopic appearance of specimens from patients with chronic ulcerative colitis and control patients was examined. Descriptive criteria were established to identify normal, atrophic, and dysplastic colonic mucosa. In the second phase, quantitative techniques were used to develop more objective criteria for the diagnosis of dysplasia in ulcerative colitis. Twenty-one coded colonic specimens from 11 patients were sequentially examined by scanning electron microscopy and by light microscopy. The three morphometric analyses performed on the surface epithelial cells were number of cells per unit area, number of microvilli per unit area, and percentage of microvilli with a normal width. The cell count and percentage of microvilli with a normal width were significantly reduced in the seven specimens with colonic dysplasia as compared with nondys-plastic tissues. Scanning electron microscopy may serve as an adjunct to light microscopy in the diagnosis of colonic dysplasia.",
author = "Shields, \{Helen M.\} and Bates, \{Margaret L.\} and Harvey Goldman and Zuckerman, \{Gary R.\} and Mills, \{Barry A.\} and Best, \{Cynthia J.\} and Bair, \{Frank A.\} and Goran, \{David A.\} and Katherine DeSchryver-Kecskemeti",
note = "Funding Information: Received February 22, 1983. Accepted January 5, 1985. Address requests for reprints to: Helen M. Shields, M.D., Division of Gastroenterology, Beth Israel Hospital, 330 Brookline Avenue, Boston, Massachusetts 02215. This work was presented in part at the annual meeting of the American Gastroenterological Association, May 21, 1984. This work was supported by a Research Grant Award from the National Foundation for Ileitis and Colitis; and in part by Grant RR-01032 from the General Clinical Research Centers Program of the Division of Research Resources, National Institutes of Health. The authors thank Dr. Rodger Haggitt for providing known dysplasia case material for analysis; Dr. Ilan Hammell and Dr. Paul Davies for their advice on the morphometric analyses; Dr. Bernard Ransil for his advice on the statistical analysis; and Dr. David Kipnis, Dr. David Alpers, and Dr. Raj Goyal for their generous support. They also thank Sybil Goulkin for her gracious help and Fran Lapidas for assistance in manuscript preparation. 0 1985 by the American Gastroenterological Association 0016-5085/85/\$3.30",
year = "1985",
month = jul,
doi = "10.1016/0016-5085(85)90746-2",
language = "English",
volume = "89",
pages = "62--72",
journal = "Gastroenterology",
issn = "0016-5085",
number = "1",
}