@article{4fd1bfac93b849e099b29e994b397f32,
title = "SC35 plays a role in T cell development and alternative splicing of CD45",
abstract = "Molecular diversity via alternative splicing is important for cellular function and development. SR proteins are strong candidate regulators of alternative splicing because they can modulate splice site selection. However, endogenous substrates for SR proteins are largely unknown, and their roles as splicing regulators in vertebrate development are unclear. Here we report that Cre-mediated conditional deletion of the prototypical SR protein SC35 in the thymus causes a defect in T cell maturation. Deletion of SC35 alters alternative splicing of CD45, a receptor tyrosine phosphatase known to be regulated by differential splicing during thymocyte development and activation. This study establishes a model to address the function of SR proteins in physiological settings and reveals a critical role of SC35 in a T cell-specific regulated splicing pathway.",
author = "Wang, {Huan You} and Xiangdong Xu and Ding, {Jian Hua} and Bermingham, {John R.} and Fu, {Xiang Dong}",
note = "Funding Information: We thank Mr. Daniel Chui and Dr. James. D. Marth of UCSD for providing numerous reagents, including a TK-specific primer, the LoxP vector, the Cre expression plasmid, probes for loxP and Cre, mouse CD45 cDNA, and Lck-Cre transgenic mice. We are grateful to Dr. Yang Xu, Dr. Steffan Ho, Dr. Rachel Soloff, Dr. Cornelis Murre, Dr. Jo Yeakley, Dr. Chi Yun, Dr. Jiwu Wang, Dr. Yang Liu, Dr. Kristen Lynch, Dr. Jim Manley, and Dr. Tom Maniatis for insightful suggestions throughout the course of this study and for critical reading of the manuscript. We are indebted to Dr. Forrest Liu of UCSD Embryonic Stem Cell Facility for ES cell transfection, selection, and microinjection. X-D. F. is a Scholar of the Leukemia and Lymphoma Society. This work was supported by a grant from National Institutes of Health to X-D. F. ",
year = "2001",
doi = "10.1016/S1097-2765(01)00181-2",
language = "English",
volume = "7",
pages = "331--342",
journal = "Molecular cell",
issn = "1097-2765",
number = "2",
}