TY - JOUR
T1 - SARS-CoV-2 requires acidic pH to infect cells
AU - Kreutzberger, Alex J.B.
AU - Sanyal, Anwesha
AU - Saminathan, Anand
AU - Bloyet, Louis Marie
AU - Stumpf, Spencer
AU - Liu, Zhuoming
AU - Ojha, Ravi
AU - Patjas, Markku T.
AU - Geneid, Ahmed
AU - Scanavachi, Gustavo
AU - Doyle, Catherine A.
AU - Somerville, Elliott
AU - Da Cunha Correia, Ricardo Bango
AU - Caprio, Giuseppe Di
AU - Toppila-Salmi, Sanna
AU - Mäkitie, Antti
AU - Kiessling, Volker
AU - Vapalahti, Olli
AU - Whelan, Sean P.J.
AU - Balistreri, Giuseppe
AU - Kirchhausen, Tom
N1 - Publisher Copyright:
Copyright © 2022 the Author(s). Published by PNAS.
PY - 2022/9/20
Y1 - 2022/9/20
N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cell entry starts with membrane attachment and ends with spike (S) protein–catalyzed membrane fusion depending on two cleavage steps, namely, one usually by furin in producing cells and the second by TMPRSS2 on target cells. Endosomal cathepsins can carry out both. Using real-time three-dimensional single-virion tracking, we show that fusion and genome penetration require virion exposure to an acidic milieu of pH 6.2 to 6.8, even when furin and TMPRSS2 cleavages have occurred. We detect the sequential steps of S1-fragment dissociation, fusion, and content release from the cell surface in TMPRRS2-overexpressing cells only when exposed to acidic pH. We define a key role of an acidic environment for successful infection, found in endosomal compartments and at the surface of TMPRSS2-expressing cells in the acidic milieu of the nasal cavity.
AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cell entry starts with membrane attachment and ends with spike (S) protein–catalyzed membrane fusion depending on two cleavage steps, namely, one usually by furin in producing cells and the second by TMPRSS2 on target cells. Endosomal cathepsins can carry out both. Using real-time three-dimensional single-virion tracking, we show that fusion and genome penetration require virion exposure to an acidic milieu of pH 6.2 to 6.8, even when furin and TMPRSS2 cleavages have occurred. We detect the sequential steps of S1-fragment dissociation, fusion, and content release from the cell surface in TMPRRS2-overexpressing cells only when exposed to acidic pH. We define a key role of an acidic environment for successful infection, found in endosomal compartments and at the surface of TMPRSS2-expressing cells in the acidic milieu of the nasal cavity.
KW - 3D imaging
KW - SARS-CoV-2
KW - infection route
KW - live-cell imaging
KW - virus entry
UR - http://www.scopus.com/inward/record.url?scp=85137109925&partnerID=8YFLogxK
U2 - 10.1073/pnas.2209514119
DO - 10.1073/pnas.2209514119
M3 - Article
C2 - 36048924
AN - SCOPUS:85137109925
SN - 0027-8424
VL - 119
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 38
M1 - e2209514119
ER -