Salvianolic acid B, an antioxidant from Salvia miltiorrhiza, prevents 6-hydroxydopamine induced apoptosis in SH-SY5Y cells

Lin Lin Tian, Xue Jun Wang, Yu Ning Sun, Chun Rong Li, Ya Ling Xing, Hai Bao Zhao, Ming Duan, Zhe Zhou, Sheng Qi Wang

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

Oxidative stress caused by dopamine may play an important role in the pathogenesis of Parkinson's disease. Salvianolic acid B is an antioxidant derived from the Chinese herb, Salvia miltiorrhiza. In this study, we investigated the neuroprotective effect of salvianolic acid B against 6-hydroxydopamine-induced cell death in human neuroblastoma SH-SY5Y cells. Pretreatment of SH-SY5Y cells with salvianolic acid B significantly reduced 6-hydroxydopamine-induced generation of reactive oxygen species, and prevented 6-hydroxydopamine-induced increases in intracellular calcium. Our data demonstrated that 6-hydroxydopamine-induced apoptosis was reversed by salvianolic acid B treatment. Salvianolic acid B reduced the 6-hydroxydopamine-induced increase of caspase-3 activity, and reduced cytochrome C translocation into the cytosol from mitochondria. The 6-hydroxydopamine-induced decrease in the Bcl-x/Bax ratio was prevented by salvianolic acid B. Additionally, salvianolic acid B decreased the activation of extracellular signal-regulated kinase and induced the activation of 6-hydroxydopamine-suppressed protein kinase C. These results indicate that the protective function of salvianolic acid B is dependent upon its antioxidative potential. Our results strongly suggest that salvianolic acid B may be effective in treating neurodegenerative diseases associated with oxidative stress.

Original languageEnglish
Pages (from-to)409-422
Number of pages14
JournalInternational Journal of Biochemistry and Cell Biology
Volume40
Issue number3
DOIs
StatePublished - Feb 18 2008
Externally publishedYes

Keywords

  • 6-Hydroxydopamine
  • Apoptosis
  • Mitogen-activated protein (MAP) kinase
  • Parkinson's disease
  • Reactive oxygen species

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