Abstract
A type III protein secretion system encoded by Salmonella pathogenicity island 2 (SPI2) has been found to be required for virulence and survival within macrophages. Here, SPI2 was shown to allow Salmonella typhimurium to avoid NADPH oxidase-dependent killing by macrophages. The ability of SPI2- mutant bacteria to survive in macrophages and to cause lethal infection in mice was restored by abrogation of the NADPH oxidase-dependent respiratory burst. Ultrastructural and immunofluorescence microscopy demonstrated efficient localization of the NADPH oxidase in the proximity of vacuoles containing. SP12-mutant but not wild-type bacteria, suggesting that SP12 interferes with trafficking of oxidase-containing vesicles to the phagosome.
Original language | English |
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Pages (from-to) | 1655-1658 |
Number of pages | 4 |
Journal | Science |
Volume | 287 |
Issue number | 5458 |
DOIs | |
State | Published - Mar 3 2000 |