Saccharomyces cerevisiae and Neurospora crassa contain heavy metal sequestering phytochelatin

Ralf Kneer; Toni M. Kutchan; Andreas Hochberger; Meinhart H. Zenk

doi:10.1007/BF00248673

Saccharomyces cerevisiae and Neurospora crassa contain heavy metal sequestering phytochelatin

Ralf Kneer, Toni M. Kutchan, Andreas Hochberger, Meinhart H. Zenk

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

In fungi, cellular resistance to heavy metal cytotoxicity is mediated either by binding of metal ions to proteins of the metallothionein type or by chelation to phytochelatin-peptides of the general formula (γ-Glu-Cys)_n-Gly. Hitherto, only one fungus, Candida glabrata has been shown to contain both metal inactivating systems. Here we show by unambiguous FAB-MS analysis that both a metallothionein-free mutant of Saccharomyces cerevisiae as well as a wildtype strain synthesize phytochelatin (PC₂) upon exposure to 250 μM Cd²⁺ ions. The presence of Zn and/or Cu ions in the nutrient broth also induces PC₂ synthesis in this organism. By ¹⁰⁹Cd exchange and subsequent monobromobimane fluorescence HPLC, it could be shown that the presence of Cd²⁺ in the growth medium also induces phytochelatin synthesis in Neurospora crassa, which contains metallothioneins.

Original language	English
Pages (from-to)	305-310
Number of pages	6
Journal	Archives of Microbiology
Volume	157
Issue number	4
DOIs	https://doi.org/10.1007/BF00248673
State	Published - Apr 1992

Keywords

Heavy metal detoxification
Metallothionein
Neurospora crassa
Phytochelatin
Saccharomyces cerevisiae

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title = "Saccharomyces cerevisiae and Neurospora crassa contain heavy metal sequestering phytochelatin",

abstract = "In fungi, cellular resistance to heavy metal cytotoxicity is mediated either by binding of metal ions to proteins of the metallothionein type or by chelation to phytochelatin-peptides of the general formula (γ-Glu-Cys)n-Gly. Hitherto, only one fungus, Candida glabrata has been shown to contain both metal inactivating systems. Here we show by unambiguous FAB-MS analysis that both a metallothionein-free mutant of Saccharomyces cerevisiae as well as a wildtype strain synthesize phytochelatin (PC2) upon exposure to 250 μM Cd2+ ions. The presence of Zn and/or Cu ions in the nutrient broth also induces PC2 synthesis in this organism. By 109Cd exchange and subsequent monobromobimane fluorescence HPLC, it could be shown that the presence of Cd2+ in the growth medium also induces phytochelatin synthesis in Neurospora crassa, which contains metallothioneins.",

keywords = "Heavy metal detoxification, Metallothionein, Neurospora crassa, Phytochelatin, Saccharomyces cerevisiae",

author = "Ralf Kneer and Kutchan, {Toni M.} and Andreas Hochberger and Zenk, {Meinhart H.}",

year = "1992",

month = apr,

doi = "10.1007/BF00248673",

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volume = "157",

pages = "305--310",

journal = "Archives of Microbiology",

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T1 - Saccharomyces cerevisiae and Neurospora crassa contain heavy metal sequestering phytochelatin

AU - Kneer, Ralf

AU - Kutchan, Toni M.

AU - Hochberger, Andreas

AU - Zenk, Meinhart H.

PY - 1992/4

Y1 - 1992/4

N2 - In fungi, cellular resistance to heavy metal cytotoxicity is mediated either by binding of metal ions to proteins of the metallothionein type or by chelation to phytochelatin-peptides of the general formula (γ-Glu-Cys)n-Gly. Hitherto, only one fungus, Candida glabrata has been shown to contain both metal inactivating systems. Here we show by unambiguous FAB-MS analysis that both a metallothionein-free mutant of Saccharomyces cerevisiae as well as a wildtype strain synthesize phytochelatin (PC2) upon exposure to 250 μM Cd2+ ions. The presence of Zn and/or Cu ions in the nutrient broth also induces PC2 synthesis in this organism. By 109Cd exchange and subsequent monobromobimane fluorescence HPLC, it could be shown that the presence of Cd2+ in the growth medium also induces phytochelatin synthesis in Neurospora crassa, which contains metallothioneins.

AB - In fungi, cellular resistance to heavy metal cytotoxicity is mediated either by binding of metal ions to proteins of the metallothionein type or by chelation to phytochelatin-peptides of the general formula (γ-Glu-Cys)n-Gly. Hitherto, only one fungus, Candida glabrata has been shown to contain both metal inactivating systems. Here we show by unambiguous FAB-MS analysis that both a metallothionein-free mutant of Saccharomyces cerevisiae as well as a wildtype strain synthesize phytochelatin (PC2) upon exposure to 250 μM Cd2+ ions. The presence of Zn and/or Cu ions in the nutrient broth also induces PC2 synthesis in this organism. By 109Cd exchange and subsequent monobromobimane fluorescence HPLC, it could be shown that the presence of Cd2+ in the growth medium also induces phytochelatin synthesis in Neurospora crassa, which contains metallothioneins.

KW - Heavy metal detoxification

KW - Metallothionein

KW - Neurospora crassa

KW - Phytochelatin

KW - Saccharomyces cerevisiae

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DO - 10.1007/BF00248673

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AN - SCOPUS:0026578156

SN - 0302-8933

VL - 157

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EP - 310

JO - Archives of Microbiology

JF - Archives of Microbiology

IS - 4

ER -

Saccharomyces cerevisiae and Neurospora crassa contain heavy metal sequestering phytochelatin

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